PMID- 22892681
OWN - NLM
STAT- MEDLINE
DCOM- 20130214
LR  - 20211021
IS  - 1473-0189 (Electronic)
IS  - 1473-0197 (Print)
IS  - 1473-0189 (Linking)
VI  - 12
IP  - 20
DP  - 2012 Oct 21
TI  - An integrated microfluidic platform for in situ cellular cytokine secretion 
      immunophenotyping.
PG  - 4093-101
AB  - Rapid, quantitative detection of cell-secreted biomarker proteins with a low 
      sample volume holds great promise to advance cellular immunophenotyping 
      techniques for personalized diagnosis and treatment of infectious diseases. Here 
      we achieved such an assay with the THP-1 human acute moncytic leukemia cell line 
      (a model for human monocyte) using a highly integrated microfluidic platform 
      incorporating a no-wash bead-based chemiluminescence immunodetection scheme. Our 
      microfluidic device allowed us to stimulate cells with lipopolysaccharide (LPS), 
      which is an endotoxin causing septic shock due to severely pronounced immune 
      response of the human body, under a well-controlled on-chip environment. Tumor 
      necrosis factor-alpha (TNF-alpha) secreted from stimulated THP-1 cells was 
      subsequently measured within the device with no flushing process required. Our 
      study achieved high-sensitivity cellular immunophenotyping with 20-fold fewer 
      cells than current cell-stimulation assay. The total assay time was also 7 times 
      shorter than that of a conventional enzyme-linked immunosorbent assay (ELISA). 
      Our strategy of monitoring immune cell functions in situ using a microfluidic 
      platform could impact future medical treatments of acute infectious diseases and 
      immune disorders by enabling a rapid, sample-efficient cellular immunophenotyping 
      analysis.
FAU - Huang, Nien-Tsu
AU  - Huang NT
AD  - Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 
      48109, USA.
FAU - Chen, Weiqiang
AU  - Chen W
FAU - Oh, Bo-Ram
AU  - Oh BR
FAU - Cornell, Timothy T
AU  - Cornell TT
FAU - Shanley, Thomas P
AU  - Shanley TP
FAU - Fu, Jianping
AU  - Fu J
FAU - Kurabayashi, Katsuo
AU  - Kurabayashi K
LA  - eng
GR  - R01 HL119542/HL/NHLBI NIH HHS/United States
GR  - R01HL097361/HL/NHLBI NIH HHS/United States
GR  - UL1RR024986/RR/NCRR NIH HHS/United States
GR  - K08 HD062142/HD/NICHD NIH HHS/United States
GR  - R01 HL097361/HL/NHLBI NIH HHS/United States
GR  - UL1 RR024986/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cell Culture Techniques/instrumentation/methods
MH  - Cell Line, Tumor
MH  - Humans
MH  - Immunophenotyping/instrumentation/*methods
MH  - Lipopolysaccharides/pharmacology
MH  - Luminescent Measurements/instrumentation/*methods
MH  - Microfluidic Analytical Techniques/instrumentation/*methods
MH  - Monocytes/cytology/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
PMC - PMC3508001
MID - NIHMS404652
EDAT- 2012/08/16 06:00
MHDA- 2013/02/15 06:00
PMCR- 2013/10/21
CRDT- 2012/08/16 06:00
PHST- 2012/08/16 06:00 [entrez]
PHST- 2012/08/16 06:00 [pubmed]
PHST- 2013/02/15 06:00 [medline]
PHST- 2013/10/21 00:00 [pmc-release]
AID - 10.1039/c2lc40619e [doi]
PST - ppublish
SO  - Lab Chip. 2012 Oct 21;12(20):4093-101. doi: 10.1039/c2lc40619e.