PMID- 22893224
OWN - NLM
STAT- MEDLINE
DCOM- 20130821
LR  - 20130304
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 97
IP  - 6
DP  - 2013 Mar
TI  - Exploration of two epimerase homologs in Streptomyces peucetius ATCC 27952.
PG  - 2493-502
LID - 10.1007/s00253-012-4327-2 [doi]
AB  - Streptomyces peucetius ATCC 27952 is a potent producer of the therapeutically 
      important antitumor drug, doxorubicin. S. peucetius contains two deoxythymidine 
      diphospho (dTDP)-4-keto-6-deoxyglucose 3,5-epimerase-encoding genes, dnmU and 
      rmbC, in its genome. While dnmU from the doxorubicin biosynthesis gene cluster is 
      involved in the biosynthesis of dTDP-L-daunosamine, rmbC is involved in the 
      biosynthesis of dTDP-L-rhamnose, a precursor of cell wall biosynthesis. The 
      proteins encoded by dnmU and rmbC share 47 % identity and 64 % similarity with 
      each other. Both enzymes converted the same substrate, 
      dTDP-4-keto-6-deoxy-D-glucose, into dTDP-4-keto-L-rhamnose in vitro. However, 
      when disruption of dnmU or rmbC was carried out, neither gene in S. peucetius 
      compensated for each other's loss of function in vivo. These results demonstrated 
      that although dnmU and rmbC encode for similar functional proteins, their native 
      roles in their respective biosynthetic pathways in vivo are specific and 
      independent of one other. Moreover, the disruption of rmbC resulted in fragmented 
      mycelia that quickly converted into gray pigmented spores. Additionally, the 
      production of doxorubicin, a major product of S. peucetius, appeared to be 
      abolished after the disruption of rmbC, demonstrating its pleiotropic effect. 
      This adverse effect might have switched on the genes encoding for spore 
      formation, arresting the expression of many genes and, thereby, preventing the 
      production of other metabolites.
FAU - Singh, Bijay
AU  - Singh B
AD  - Department of Pharmaceutical Engineering, Institute of Biomolecule 
      Reconstruction, SunMoon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, 
      Chungnam 336-708, Republic of Korea.
FAU - Oh, Tae Jin
AU  - Oh TJ
FAU - Sohng, Jae Kyung
AU  - Sohng JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120816
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - EC 5.1.3.- (Carbohydrate Epimerases)
RN  - EC 5.1.3.- (dTDP-4-keto-6-deoxyglucose-5-epimerase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carbohydrate Epimerases/*genetics/*metabolism
MH  - Gene Deletion
MH  - Gene Order
MH  - Genetic Complementation Test
MH  - Microscopy
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Streptomyces/cytology/*enzymology/*genetics/growth & development
EDAT- 2012/08/16 06:00
MHDA- 2013/08/22 06:00
CRDT- 2012/08/16 06:00
PHST- 2012/05/29 00:00 [received]
PHST- 2012/07/18 00:00 [accepted]
PHST- 2012/07/03 00:00 [revised]
PHST- 2012/08/16 06:00 [entrez]
PHST- 2012/08/16 06:00 [pubmed]
PHST- 2013/08/22 06:00 [medline]
AID - 10.1007/s00253-012-4327-2 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2013 Mar;97(6):2493-502. doi: 
      10.1007/s00253-012-4327-2. Epub 2012 Aug 16.