PMID- 22893508
OWN - NLM
STAT- MEDLINE
DCOM- 20130207
LR  - 20191210
IS  - 1615-9314 (Electronic)
IS  - 1615-9306 (Linking)
VI  - 35
IP  - 19
DP  - 2012 Oct
TI  - Quantification of ampicillin in bovine milk by coupled-column 
      ultrahigh-performance liquid chromatography-tandem mass spectrometry.
PG  - 2615-20
LID - 10.1002/jssc.201200205 [doi]
AB  - This work reports the use of two-dimensional (2D) liquid chromatography system 
      coupled with a tandem mass spectrometry for the quantification of ampicillin in 
      bovine milk. A restrict access media column (RAM-BSA C(8) , 50 x 2.1 mm, Luna, 10 
      mum, 100 A) was used in the first dimension in order to exclude macromolecules, 
      while an ACQUITY UPLC BEH C(18) (50 x 2.1 mm, 1.7 mum) column was used in the 
      second dimension. Three different channels of selected reaction monitoring (SRM) 
      were used: 350 > 106 m/z, 350 > 160 m/z, and 350 > 192 m/z. The first transition 
      was used for the quantification (higher intensity), and latter two for 
      confirmation. The developed method is simple and requires a total analysis time 
      of only 14 min/sample. The sample treatment involved only a centrifugation step 
      for 20 min. The validated method has been successfully applied to monitor AMP 
      residues in raw milk samples. To our knowledge, this is the first study to report 
      the use of ultrahigh-performance liquid chromatography (UHPLC) in 2D 
      configuration.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Moura, Franciane
AU  - Moura F
AD  - Department of Chemistry, Federal University of Sao Carlos, Sao Carlos, Sao Paulo, 
      Brazil.
FAU - de Almeida, Fernando G
AU  - de Almeida FG
FAU - Lopes, Bianca Rebelo
AU  - Lopes BR
FAU - Cass, Quezia Bezerra
AU  - Cass QB
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20120815
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Anti-Bacterial Agents)
RN  - 7C782967RD (Ampicillin)
SB  - IM
MH  - Ampicillin/*analysis
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Cattle
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Food Contamination/analysis
MH  - Milk/*chemistry
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/08/16 06:00
MHDA- 2013/02/08 06:00
CRDT- 2012/08/16 06:00
PHST- 2012/02/26 00:00 [received]
PHST- 2012/04/24 00:00 [revised]
PHST- 2012/05/29 00:00 [accepted]
PHST- 2012/08/16 06:00 [entrez]
PHST- 2012/08/16 06:00 [pubmed]
PHST- 2013/02/08 06:00 [medline]
AID - 10.1002/jssc.201200205 [doi]
PST - ppublish
SO  - J Sep Sci. 2012 Oct;35(19):2615-20. doi: 10.1002/jssc.201200205. Epub 2012 Aug 
      15.