PMID- 22893639
OWN - NLM
STAT- MEDLINE
DCOM- 20140616
LR  - 20121203
IS  - 0027-5514 (Print)
IS  - 0027-5514 (Linking)
VI  - 104
IP  - 6
DP  - 2012 Nov-Dec
TI  - Improved detection and identification of aquatic fungi and chitin in aquatic 
      environments.
PG  - 1267-71
LID - 10.3852/11-225 [doi]
AB  - Chitin is a polymer of major importance in aquatic environments. We report on a 
      new chitin-staining method for environmental samples. Major advantages of this 
      method are (i) its high specificity and (ii) its suitability for combination with 
      general stains, such as 4',6-diamidino-2-phenylindole (DAPI), or taxa-specific 
      methods, such as fluorescence in situ hybridization (FISH). This method lets 
      aquatic mycologists explore basal fungal lineages directly in situ, identify 
      their hosts, occurrence and evolutionary traits.
FAU - Wurzbacher, Christian
AU  - Wurzbacher C
AD  - Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Dept. Limnology of 
      Stratified Lakes, Stechlin, Germany.
FAU - Grossart, Hans-Peter
AU  - Grossart HP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120814
PL  - England
TA  - Mycologia
JT  - Mycologia
JID - 0400764
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 1398-61-4 (Chitin)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Biofilms
MH  - Cell Wall/ultrastructure
MH  - Chitin/*isolation & purification
MH  - Fluorescent Dyes
MH  - Fungi/*isolation & purification/ultrastructure
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Indoles
MH  - Microscopy, Fluorescence
MH  - Sensitivity and Specificity
MH  - Sporangia/ultrastructure
MH  - Yeasts/ultrastructure
EDAT- 2012/08/16 06:00
MHDA- 2014/06/17 06:00
CRDT- 2012/08/16 06:00
PHST- 2012/08/16 06:00 [entrez]
PHST- 2012/08/16 06:00 [pubmed]
PHST- 2014/06/17 06:00 [medline]
AID - 11-225 [pii]
AID - 10.3852/11-225 [doi]
PST - ppublish
SO  - Mycologia. 2012 Nov-Dec;104(6):1267-71. doi: 10.3852/11-225. Epub 2012 Aug 14.