PMID- 22895527
OWN - NLM
STAT- MEDLINE
DCOM- 20130307
LR  - 20161125
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 30
IP  - 5
DP  - 2012 Nov
TI  - Evaluation of the interactions of HIV-1 integrase with small ubiquitin-like 
      modifiers and their conjugation enzyme Ubc9.
PG  - 1053-60
LID - 10.3892/ijmm.2012.1088 [doi]
AB  - Human immunodeficiency virus type 1 (HIV-1) integrase mediates the integration of 
      reverse-transcribed viral cDNA into the genome of the host for the stable 
      maintenance of the viral genome and the persistence of HIV-1 infection. In this 
      study, the relationships between HIV-1 integrase (HIV-1 IN) and three SUMO 
      conjugation pathway proteins, as well as the effects of these associations, were 
      investigated. The overexpression of SUMO1/SUMO2 and Ubc9 changed the 
      intracellular localization of HIV-1 IN from a diffuse distribution to a punctate 
      localization. SUMO1, SUMO2 and Ubc9 were shown to interact with HIV-1 IN. The 
      SUMOylation of HIV-1 IN was verified. In addition, SUMO1, SUMO2 and Ubc9 were 
      shown to influence the integration of both lentivirus and HIV-1. The 
      overexpression of Ubc9 inhibited viral genome integration, and the upregulation 
      of SUMO1 or SUMO2 enhanced the inhibitory effect of Ubc9. Knockdown of the 
      endogenous levels of SUMO1, SUMO2 and Ubc9 increased the level of viral 
      integration, while reverse transcription and the nuclear import of preintegration 
      complex (PIC) were not affected. Our findings suggest that SUMO conjugation 
      pathway proteins may act as cellular restriction factors and be detrimental to 
      HIV-1 infection. These findings merit further investigation because of their 
      potentially significant implications for the cellular antiviral response to HIV-1 
      infection.
FAU - Li, Zhihui
AU  - Li Z
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of 
      Life Sciences, Fudan University, Shanghai 200433, PR China.
FAU - Wu, Shuwen
AU  - Wu S
FAU - Wang, Jingjing
AU  - Wang J
FAU - Li, Wenjuan
AU  - Li W
FAU - Lin, Yun
AU  - Lin Y
FAU - Ji, Chaoneng
AU  - Ji C
FAU - Xue, Jinglun
AU  - Xue J
FAU - Chen, Jinzhong
AU  - Chen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120808
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (SUMO-1 Protein)
RN  - 0 (SUMO1 protein, human)
RN  - 0 (SUMO2 protein, human)
RN  - 0 (Small Ubiquitin-Related Modifier Proteins)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 2.7.7.- (HIV Integrase)
RN  - EC 6.3.2.- (ubiquitin-conjugating enzyme UBC9)
SB  - IM
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - HEK293 Cells
MH  - HIV Integrase/*metabolism
MH  - HIV-1/*enzymology/physiology
MH  - Humans
MH  - Lentivirus/physiology
MH  - Protein Binding
MH  - Protein Processing, Post-Translational
MH  - Protein Transport
MH  - RNA Interference
MH  - SUMO-1 Protein/genetics/*metabolism
MH  - Small Ubiquitin-Related Modifier Proteins/genetics/*metabolism
MH  - Sumoylation
MH  - Two-Hybrid System Techniques
MH  - Ubiquitin-Conjugating Enzymes/genetics/*metabolism
MH  - Virus Integration
EDAT- 2012/08/17 06:00
MHDA- 2013/03/08 06:00
CRDT- 2012/08/17 06:00
PHST- 2012/03/21 00:00 [received]
PHST- 2012/05/18 00:00 [accepted]
PHST- 2012/08/17 06:00 [entrez]
PHST- 2012/08/17 06:00 [pubmed]
PHST- 2013/03/08 06:00 [medline]
AID - 10.3892/ijmm.2012.1088 [doi]
PST - ppublish
SO  - Int J Mol Med. 2012 Nov;30(5):1053-60. doi: 10.3892/ijmm.2012.1088. Epub 2012 Aug 
      8.