PMID- 22895623
OWN - NLM
STAT- MEDLINE
DCOM- 20130612
LR  - 20131121
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 350
IP  - 2
DP  - 2012 Nov
TI  - A new convenient technique for making cell blocks.
PG  - 395-400
LID - 10.1007/s00441-012-1467-8 [doi]
AB  - Cell block sections serve as an important diagnostic annex for cytological 
      smears, liquid-based SurePath cytology and the Liquid-based Thin-prep Cytology 
      Test (TCT). A variety of methods for the preparation of cell blocks are described 
      in the literature and the techniques in cell blocks are in continuous 
      improvement. A new technique for making cell blocks was introduced in the present 
      study. We first used pregelatinized starch as the frame for the cell block, which 
      is a really simple and economic method, because it can be carried out at room 
      temperature without additional special instruments. We have performed hematoxylin 
      and eosin (HE) staining, immunohistochemistry analysis and fluorescence in situ 
      hybridization (FISH) in the cell block sections in 122 cytological specimens. The 
      results demonstrated in this article show that pregelatinized starch is a useful 
      frame for cell blocks. The pregelatinized starch can effectively collect even a 
      few cells with powerful adhesiveness. Therefore, this new technique for making 
      cell blocks is especially useful for cytologic samples with low cellularity, such 
      as cerebrospinal fluid specimens.
FAU - He, Qing-Lian
AU  - He QL
AD  - Department of Pathology, Guangdong Provincial Hospital of TCM, Guangzhou 
      University of Chinese Medicine, 111 Dade Road, Guangzhou, 510120, China. 
      28905709@qq.com
FAU - Zhu, Ya-Zhen
AU  - Zhu YZ
FAU - Zheng, Guang-Juan
AU  - Zheng GJ
FAU - Shi, Ling-Chun
AU  - Shi LC
FAU - Hu, Shao-Wei
AU  - Hu SW
FAU - Li, Chu-Tian
AU  - Li CT
LA  - eng
PT  - Journal Article
DEP - 20120816
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - YKM8PY2Z55 (Hematoxylin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cytological Techniques/*instrumentation/*methods
MH  - Female
MH  - Hematoxylin/chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Young Adult
EDAT- 2012/08/17 06:00
MHDA- 2013/06/13 06:00
CRDT- 2012/08/17 06:00
PHST- 2012/03/27 00:00 [received]
PHST- 2012/06/20 00:00 [accepted]
PHST- 2012/08/17 06:00 [entrez]
PHST- 2012/08/17 06:00 [pubmed]
PHST- 2013/06/13 06:00 [medline]
AID - 10.1007/s00441-012-1467-8 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2012 Nov;350(2):395-400. doi: 10.1007/s00441-012-1467-8. Epub 
      2012 Aug 16.