PMID- 22895657
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20130604
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 41
IP  - 4
DP  - 2012 Oct
TI  - Biological influence of brain-derived neurotrophic factor on breast cancer cells.
PG  - 1541-6
LID - 10.3892/ijo.2012.1581 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin 
      superfamily which has been indicated in the pathophysiology of the nervous system 
      and is important in a number of neurological and psychological conditions. 
      Recently, BDNF was also shown to play a role in the development and progression 
      of solid tumour myeloma. It has been reported that BDNF is aberrantly expressed 
      in human breast cancer and that a raised level of BDNF is associated with poor 
      clinical outcome and reduced survival. The present study investigated the role of 
      BDNF in human breast cancer. A panel of human breast cancer cells was used and 
      the expression profile of BDNF was evaluated using RT-PCR. We constructed a set 
      of anti-BDNF transgenes which were used to transfect breast cancer cells in order 
      to generate BDNF knocked down cells. The impact of BDNF knockdown on growth and 
      apoptosis was evaluated. Statistical analysis was performed using SPSS. P<0.05 
      was considered statistically significant. BDNF gene transcripts were successfully 
      detected in the breast cancer cell lines MCF-7, MDA-MB-231 and ZR75-1 MDA-MB-231 
      and MCF-7 wild-type cells were subject to transfection of anti-BDNF transgenes, 
      followed by the establishment of BDNF knocked down sublines. Knockdown of BDNF in 
      MDA-MB-231 and MCF-7 cell lines resulted in decreased rates of growth and 
      proliferation. Analysis of apoptosis showed that cell apoptosis was increased in 
      cells stably transfected with ribozymes for BDNF compared with the vector 
      control. It is concluded that BDNF, a neurotrophic growth factor aberrantly 
      expressed in cancers such as breast cancer, has a profound impact on the cellular 
      behaviour of breast cancer cells and that BDNF is associated with a reduction of 
      the apoptosis of breast cancer. BDNF is, therefore, a potential therapeutic 
      target in breast cancer and its effect in human breast cancer requires further 
      investigation.
FAU - Yang, Xiaomei
AU  - Yang X
AD  - Metastasis and Angiogenesis Research Group, Institute of Cancer and Genetics, 
      Cardiff University School of Medicine, Cardiff, CF14 4XN, UK.
FAU - Martin, Tracey A
AU  - Martin TA
FAU - Jiang, Wen G
AU  - Jiang WG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120806
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - MCF-7 Cells
MH  - Signal Transduction/genetics
MH  - Transgenes
EDAT- 2012/08/17 06:00
MHDA- 2014/01/01 06:00
CRDT- 2012/08/17 06:00
PHST- 2012/04/19 00:00 [received]
PHST- 2012/06/15 00:00 [accepted]
PHST- 2012/08/17 06:00 [entrez]
PHST- 2012/08/17 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - 10.3892/ijo.2012.1581 [doi]
PST - ppublish
SO  - Int J Oncol. 2012 Oct;41(4):1541-6. doi: 10.3892/ijo.2012.1581. Epub 2012 Aug 6.