PMID- 22898896
OWN - NLM
STAT- MEDLINE
DCOM- 20130322
LR  - 20211021
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Print)
IS  - 1234-1983 (Linking)
VI  - 53
IP  - 4
DP  - 2012 Nov
TI  - Expression of human growth hormone in the milk of transgenic rabbits with 
      transgene mapped to the telomere region of chromosome 7q.
PG  - 435-42
LID - 10.1007/s13353-012-0110-4 [doi]
AB  - The advent of transgenic technology has provided methods for the production of 
      pharmaceuticals by the isolation of these proteins from transgenic animals. The 
      mammary gland has been focused on as a bioreactor, since milk is easily collected 
      from lactating animals and protein production can be expressed at very high 
      levels, including hormones and enzymes. We demonstrate here the expression 
      pattern of recombinant human growth hormone (rhGH) in transgenic rabbits carrying 
      hGH genomic sequences driven by the rat whey acidic protein (WAP) promoter. The 
      transgene was mapped to the q26-27 telomere region of chromosome 7q by 
      fluorescence in situ hybridization (FISH). Nearly 30 % of the F1 generation 
      demonstrated the presence of transgene. The recombinant growth hormone was 
      detected in the milk of the transgenic rabbit females, but not in serum, up to 
      the level of 10 mug/ml. Ectopic expression of the transgene in the brain, heart, 
      kidney, liver, and salivary gland was not observed, indicating that a short 
      sequence of rat WAP promoter (969 bp) contained essential sequences directing 
      expression exclusively to the mammary gland. The biological activity of 
      recombinant growth hormone was measured by immunoreactivity and the capability to 
      stimulate growth of the hormone-dependent Nb211 cell line.
FAU - Lipinski, Daniel
AU  - Lipinski D
AD  - Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, 
      Dojazd 11, 60-632, Poznan, Poland.
FAU - Zeyland, Joanna
AU  - Zeyland J
FAU - Szalata, Marlena
AU  - Szalata M
FAU - Plawski, Andrzej
AU  - Plawski A
FAU - Jarmuz, Malgorzata
AU  - Jarmuz M
FAU - Jura, Jacek
AU  - Jura J
FAU - Korcz, Aleksandra
AU  - Korcz A
FAU - Smorag, Zdzislaw
AU  - Smorag Z
FAU - Pienkowski, Marek
AU  - Pienkowski M
FAU - Slomski, Ryszard
AU  - Slomski R
LA  - eng
PT  - Journal Article
DEP - 20120817
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (Milk Proteins)
RN  - 0 (whey acidic proteins)
RN  - 12629-01-5 (Human Growth Hormone)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified/genetics/*metabolism
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Mammalian
MH  - Female
MH  - Gene Expression Regulation
MH  - Human Growth Hormone/*biosynthesis/genetics
MH  - Humans
MH  - Immunoradiometric Assay
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mammary Glands, Animal/chemistry
MH  - Milk/*chemistry
MH  - Milk Proteins/genetics
MH  - Promoter Regions, Genetic
MH  - Rabbits
MH  - Rats
MH  - Telomere/*genetics
MH  - *Transgenes
PMC - PMC3477484
EDAT- 2012/08/18 06:00
MHDA- 2013/03/23 06:00
PMCR- 2012/08/17
CRDT- 2012/08/18 06:00
PHST- 2012/03/16 00:00 [received]
PHST- 2012/07/30 00:00 [accepted]
PHST- 2012/06/29 00:00 [revised]
PHST- 2012/08/18 06:00 [entrez]
PHST- 2012/08/18 06:00 [pubmed]
PHST- 2013/03/23 06:00 [medline]
PHST- 2012/08/17 00:00 [pmc-release]
AID - 110 [pii]
AID - 10.1007/s13353-012-0110-4 [doi]
PST - ppublish
SO  - J Appl Genet. 2012 Nov;53(4):435-42. doi: 10.1007/s13353-012-0110-4. Epub 2012 
      Aug 17.