PMID- 22899008
OWN - NLM
STAT- MEDLINE
DCOM- 20130122
LR  - 20120914
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Linking)
VI  - 4
IP  - 19
DP  - 2012 Sep 28
TI  - Real time protein recognition in a liquid-gated carbon nanotube field-effect 
      transistor modified with aptamers.
PG  - 5917-23
LID - 10.1039/c2nr31257c [doi]
AB  - The combination of optimized and passivated Field Effect Transistors (FETs) based 
      on carbon nanotubes (CNTs) together with the appropriate choice and 
      immobilization strategy of aptamer receptors and buffer concentration have 
      allowed the highly sensitive and real time biorecognition of proteins in a 
      liquid-gated configuration. Specifically we have followed the biorecognition 
      process of thrombin by its specific aptamer. The aptamer modified device is 
      sensitive enough to capture a change in the electronic detection mechanism, one 
      operating at low protein concentrations and the other in a higher target 
      concentration range. The high sensitivity of the device is also sustained by the 
      very low detection limits achieved (20 pM) and their high selectivity when other 
      target proteins are used. Moreover, the experimental results have allowed us to 
      quantify the equilibrium constant of the protein-aptamer binding and confirm its 
      high affinity by using the Langmuir equation.
FAU - Pacios, Merce
AU  - Pacios M
AD  - Grup de Sensors i Biosensors, Departament de Quimica, Facultat de Ciencies, 
      Edifici C-nord, UniversitatAutonoma de Barcelona, 08193 Bellaterra (Barcelona), 
      Spain.
FAU - Martin-Fernandez, Inigo
AU  - Martin-Fernandez I
FAU - Borrise, Xavier
AU  - Borrise X
FAU - del Valle, Manel
AU  - del Valle M
FAU - Bartroli, Jordi
AU  - Bartroli J
FAU - Lora-Tamayo, Emilio
AU  - Lora-Tamayo E
FAU - Godignon, Philippe
AU  - Godignon P
FAU - Perez-Murano, Francesc
AU  - Perez-Murano F
FAU - Esplandiu, Maria Jose
AU  - Esplandiu MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120817
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Myoglobin)
RN  - 0 (Nanotubes, Carbon)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Myoglobin/chemistry/metabolism
MH  - Nanotubes, Carbon/*chemistry
MH  - Pancreatic Elastase/chemistry/metabolism
MH  - Thrombin/*chemistry/metabolism
MH  - *Transistors, Electronic
EDAT- 2012/08/18 06:00
MHDA- 2013/01/23 06:00
CRDT- 2012/08/18 06:00
PHST- 2012/08/18 06:00 [entrez]
PHST- 2012/08/18 06:00 [pubmed]
PHST- 2013/01/23 06:00 [medline]
AID - 10.1039/c2nr31257c [doi]
PST - ppublish
SO  - Nanoscale. 2012 Sep 28;4(19):5917-23. doi: 10.1039/c2nr31257c. Epub 2012 Aug 17.