PMID- 22900050
OWN - NLM
STAT- MEDLINE
DCOM- 20130322
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 8
DP  - 2012
TI  - GroEL1, from Chlamydia pneumoniae, induces vascular adhesion molecule 1 
      expression by p37(AUF1) in endothelial cells and hypercholesterolemic rabbit.
PG  - e42808
LID - 10.1371/journal.pone.0042808 [doi]
LID - e42808
AB  - The expression of vascular adhesion molecule-1 (VCAM-1) by endothelial cells may 
      play a major role in atherogenesis. The actual mechanisms of chlamydia pneumoniae 
      (C. pneumoniae) relate to atherogenesis are unclear. We investigate the influence 
      of VCAM-1 expression in the GroEL1 from C. pneumoniae-administered human coronary 
      artery endothelial cells (HCAECs) and hypercholesterolemic rabbits. In this 
      study, we constructed the recombinant GroEL1 from C. pneumoniae. The HCAECs/THP-1 
      adhesion assay, tube formation assay, western blotting, enzyme-linked 
      immunosorbent assay, actinomycin D chase experiment, luciferase reporter assay, 
      and immunohistochemical stainings were performed. The results show that GroEL1 
      increased both VCAM-1 expression and THP-1 cell adhesives, and impaired 
      tube-formation capacity in the HCAECs. GroEL1 significantly increased the VCAM-1 
      mRNA stability and cytosolic AU-binding factor 1 (AUF1) level. Overexpression of 
      the p37(AUF1) significantly increased VCAM-1 gene expression in GroEL1-induced 
      bovine aortic endothelial cells (BAECs). GroEL1 prolonged the stability of VCAM-1 
      mRNA by increasing both p37(AUF1) and the regulation of the 5' untranslated 
      region (UTR) of the VCAM-1 mRNA in BAECs. In hypercholesterolemic rabbits, GroEL1 
      administration enhanced fatty-streak and macrophage infiltration in 
      atherosclerotic lesions, which may be mediated by elevated VCAM-1 expression. In 
      conclusion, GroEL1 induces VCAM-1 expression by p37(AUF1) in endothelial cells 
      and enhances atherogenesis in hypercholesterolemic rabbits.
FAU - Huang, Chun-Yao
AU  - Huang CY
AD  - Department of Internal Medicine, School of Medicine, College of Medicine, Taipei 
      Medical University, Taipei, Taiwan.
FAU - Shih, Chun-Ming
AU  - Shih CM
FAU - Tsao, Nai-Wen
AU  - Tsao NW
FAU - Chen, Yung-Hsiang
AU  - Chen YH
FAU - Li, Chi-Yuan
AU  - Li CY
FAU - Chang, Yu-Jia
AU  - Chang YJ
FAU - Chang, Nen-Chung
AU  - Chang NC
FAU - Ou, Keng-Liang
AU  - Ou KL
FAU - Lin, Cheng-Yen
AU  - Lin CY
FAU - Lin, Yi-Wen
AU  - Lin YW
FAU - Nien, Chih-Hao
AU  - Nien CH
FAU - Lin, Feng-Yen
AU  - Lin FY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120810
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Chaperonin 60)
RN  - 0 (HNRNPD protein, human)
RN  - 0 (Heterogeneous Nuclear Ribonucleoprotein D0)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoprotein D)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
SB  - IM
EIN - PLoS One. 2012;7(8). doi: 10.1371/annotation/6f8adaaa-19d8-4187-9418-24a05ae77c8c
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Aorta/drug effects/metabolism
MH  - Atherosclerosis/genetics/metabolism
MH  - Cattle
MH  - Cell Line
MH  - Chaperonin 60/*metabolism/pharmacology
MH  - Chlamydophila pneumoniae/*metabolism
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Heterogeneous Nuclear Ribonucleoprotein D0
MH  - Heterogeneous-Nuclear Ribonucleoprotein D/*metabolism
MH  - Hypercholesterolemia/*genetics/*metabolism
MH  - Macrophages/immunology/metabolism
MH  - Male
MH  - RNA Stability/drug effects
MH  - Rabbits
MH  - Recombinant Proteins/metabolism/pharmacology
MH  - Vascular Cell Adhesion Molecule-1/*genetics/metabolism
PMC - PMC3416774
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/08/18 06:00
MHDA- 2013/03/23 06:00
PMCR- 2012/08/10
CRDT- 2012/08/18 06:00
PHST- 2012/03/16 00:00 [received]
PHST- 2012/07/11 00:00 [accepted]
PHST- 2012/08/18 06:00 [entrez]
PHST- 2012/08/18 06:00 [pubmed]
PHST- 2013/03/23 06:00 [medline]
PHST- 2012/08/10 00:00 [pmc-release]
AID - PONE-D-12-07975 [pii]
AID - 10.1371/journal.pone.0042808 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(8):e42808. doi: 10.1371/journal.pone.0042808. Epub 2012 Aug 10.