PMID- 22900357
OWN - NLM
STAT- MEDLINE
DCOM- 20120918
LR  - 20131121
IS  - 0301-1208 (Print)
IS  - 0301-1208 (Linking)
VI  - 41
IP  - 6
DP  - 2004 Dec
TI  - cAMP-mediated upregulation of gelatinases in primary cultures of isolated rat 
      hepatocytes.
PG  - 294-8
AB  - Matrix metalloproteinases (MMPs) play a major role in tissue remodelling and 
      repair in pathophysiological conditions, such as liver fibrosis and regeneration. 
      Regulation of the MMPs produced by liver cells is important in maintaining 
      cell-matrix ratio in liver, which is a major target site for hormones that 
      mediate their intracellular effects through cAMP. The possibility of cAMP 
      affecting the activity of MMPs and their endogenous inhibitors, tissue inhibitor 
      of MMPs (TIMPs) was studied using isolated rat hepatocytes in culture. 
      Zymographic analysis showed that treatment with hormones like epinephrine, 
      thyroxine and dexamethasone and Bt2 cAMP increased 92 kDa MMP-9 activity. Bt2 
      cAMP caused upregulation of MMP-9 in a dose-dependent manner. The effect of 
      hormones was less on MMP-2. ELISA using specific antibodies showed increase in 
      levels of MMP-9 and TIMP-1 protein. Kinetic analysis of production of MMPs and 
      TIMPs showed that the response to Bt2 cAMP was a delayed one, indicating its 
      effect on de novo protein synthesis. These results suggest the possibility of 
      cAMP dependent regulation of MMP-9 in the hepatocytes.
FAU - Saja, K
AU  - Saja K
AD  - Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram 
      695581, India.
FAU - Sudhakaran, P R
AU  - Sudhakaran PR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Indian J Biochem Biophys
JT  - Indian journal of biochemistry & biophysics
JID - 0310774
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cyclic AMP/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Extracellular Matrix/metabolism
MH  - *Gene Expression Regulation, Enzymologic
MH  - Hepatocytes/*enzymology
MH  - Horseradish Peroxidase/metabolism
MH  - Kinetics
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Matrix Metalloproteinases/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - *Up-Regulation
EDAT- 2004/12/01 00:00
MHDA- 2012/09/19 06:00
CRDT- 2012/08/21 06:00
PHST- 2012/08/21 06:00 [entrez]
PHST- 2004/12/01 00:00 [pubmed]
PHST- 2012/09/19 06:00 [medline]
PST - ppublish
SO  - Indian J Biochem Biophys. 2004 Dec;41(6):294-8.