PMID- 22900891
OWN - NLM
STAT- MEDLINE
DCOM- 20130131
LR  - 20120910
IS  - 1750-3841 (Electronic)
IS  - 0022-1147 (Linking)
VI  - 77
IP  - 9
DP  - 2012 Sep
TI  - Separation of phospholipids from hen egg yolk by short packed silica gel column 
      chromatography.
PG  - C948-53
LID - 10.1111/j.1750-3841.2012.02850.x [doi]
AB  - Short packed silica gel column chromatography has been performed to optimize the 
      production of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) from hen 
      egg yolk with very low or no toxic solvents. The effects of silica type, sample 
      loading amount, dimension of the glass chromatotube, and mobile phase 
      compositions were investigated and high separation efficiency was achieved: 
      gradient elution as 200 mL ethanol followed by 300 mL 95% ethanol to fractionate 
      PE and PC after neutral lipids (NL) removed by 120 mL ethyl acetate, 40 mm silica 
      gel (54 to 74 mum) bed height of the chromatotube with 22 mm inner dia (ID), and 
      0.25 g sample loading amount. By this procedure, 3.69 g PE and 2.88 g PC per 100 
      g egg yolk lipids were obtained, respectively. The refined PE and PC were 
      identified by high-performance liquid chromatography/ultraviolet detector 
      (HPLC-UV) with purity over 96%. The fatty acids in egg yolk revealed that PE and 
      PC characterized higher ratios of n- 6/n- 3 (PE, 7.41; PC, 8.99). 18:2 n- 6 of PC 
      (15.21%) predominated over PE (10.29%), whereas the level of 20:4 n- 6 of PC 
      (8.78%) was lower than PE (15.67%).
CI  - (c) 2012 Institute of Food Technologists(R)
FAU - Lei, Lin
AU  - Lei L
AD  - State Key Lab of Food Science and Technology, Inst. for Advanced Study, Nanchang 
      Univ, Nanchang, Jiangxi 330047, China.
FAU - Li, Jing
AU  - Li J
FAU - Hu, Jiang-ning
AU  - Hu JN
FAU - Liu, Rong
AU  - Liu R
FAU - Fan, Ya-wei
AU  - Fan YW
FAU - Tang, Liang
AU  - Tang L
FAU - Deng, Ze-yuan
AU  - Deng ZY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120817
PL  - United States
TA  - J Food Sci
JT  - Journal of food science
JID - 0014052
RN  - 0 (Fatty Acids)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Solvents)
RN  - 39382-08-6 (phosphatidylethanolamine)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Chromatography, Gel/instrumentation/*methods
MH  - Chromatography, High Pressure Liquid/methods
MH  - Egg Yolk/*chemistry
MH  - Fatty Acids/analysis
MH  - Phosphatidylcholines/chemistry/*isolation & purification
MH  - Phosphatidylethanolamines/chemistry/*isolation & purification
MH  - Silicon Dioxide
MH  - Solvents
EDAT- 2012/08/21 06:00
MHDA- 2013/02/01 06:00
CRDT- 2012/08/21 06:00
PHST- 2012/08/21 06:00 [entrez]
PHST- 2012/08/21 06:00 [pubmed]
PHST- 2013/02/01 06:00 [medline]
AID - 10.1111/j.1750-3841.2012.02850.x [doi]
PST - ppublish
SO  - J Food Sci. 2012 Sep;77(9):C948-53. doi: 10.1111/j.1750-3841.2012.02850.x. Epub 
      2012 Aug 17.