PMID- 22901507
OWN - NLM
STAT- MEDLINE
DCOM- 20121210
LR  - 20131121
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Linking)
VI  - 252
IP  - 1-2
DP  - 2012 Nov 15
TI  - Use of engineered bone marrow stem cells to deliver brain derived neurotrophic 
      factor under the control of a tetracycline sensitive response element in 
      experimental allergic encephalomyelitis.
PG  - 1-15
LID - S0165-5728(12)00217-2 [pii]
LID - 10.1016/j.jneuroim.2012.07.005 [doi]
AB  - Brain derived neurotrophic factor (BDNF) has neuroprotective properties but its 
      use has been limited by poor penetration of the blood brain barrier. Treatment 
      using bone marrow stem cells (BMSC) or retroviruses as vectors reduces the 
      clinical and pathological severity of experimental allergic encephalomyelitis 
      (EAE). We have refined the BMSC based delivery system by introducing a 
      tetracycline sensitive response element to control BDNF expression. We have now 
      tested that construct in EAE and have shown a reduction in both the clinical and 
      pathological severity of the disease. Further, we looked for changes in sirtuin1 
      and nicotinamide phosphoribosyltransferase expression that would be consistent 
      with a neuroprotective effect.
CI  - Published by Elsevier B.V.
FAU - Makar, Tapas K
AU  - Makar TK
AD  - VA Maryland Healthcare System, Baltimore, MD 21201, United States.
FAU - Nimmagadda, Vamshi K C
AU  - Nimmagadda VK
FAU - Patibandla, Gopi K
AU  - Patibandla GK
FAU - Le, Toby
AU  - Le T
FAU - Judge, Susan I V
AU  - Judge SI
FAU - Trisler, David
AU  - Trisler D
FAU - Bever, Christopher T
AU  - Bever CT
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120817
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*methods
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Encephalomyelitis, Autoimmune, Experimental/genetics/pathology/*therapy
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation
MH  - *Gene Transfer Techniques
MH  - Genetic Engineering
MH  - Genetic Vectors/pharmacology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Real-Time Polymerase Chain Reaction
MH  - Response Elements/genetics
MH  - Spinal Cord/pathology
MH  - Stem Cell Transplantation/*methods
MH  - Tetracycline/pharmacology
EDAT- 2012/08/21 06:00
MHDA- 2012/12/12 06:00
CRDT- 2012/08/21 06:00
PHST- 2012/02/13 00:00 [received]
PHST- 2012/06/29 00:00 [revised]
PHST- 2012/07/17 00:00 [accepted]
PHST- 2012/08/21 06:00 [entrez]
PHST- 2012/08/21 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - S0165-5728(12)00217-2 [pii]
AID - 10.1016/j.jneuroim.2012.07.005 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2012 Nov 15;252(1-2):1-15. doi: 10.1016/j.jneuroim.2012.07.005. 
      Epub 2012 Aug 17.