PMID- 22902328 OWN - NLM STAT- MEDLINE DCOM- 20131210 LR - 20211021 IS - 1873-4847 (Electronic) IS - 0955-2863 (Print) IS - 0955-2863 (Linking) VI - 24 IP - 5 DP - 2013 May TI - Retinoic acid receptors move in time with the clock in the hippocampus. Effect of a vitamin-A-deficient diet. PG - 859-67 LID - S0955-2863(12)00148-9 [pii] LID - 10.1016/j.jnutbio.2012.05.006 [doi] AB - An endogenous time-keeping mechanism controls circadian biological rhythms in mammals. Previously, we showed that vitamin A deficiency modifies clock BMAL1 and PER1 as well as BDNF and neurogranin daily rhythmicity in the rat hippocampus when animals are maintained under 12-h-light:12-h-dark conditions. Retinoic acid nuclear receptors, retinoic acid receptors (RARs) and retinoid X receptors (RXRs), have been detected in the same brain area. Our objectives were (a) to analyze whether RARalpha, RARbeta and RXRbeta exhibit a circadian variation in the rat hippocampus and (b) to investigate the effect of a vitamin-A-deficient diet on the circadian expression of BMAL1, PER1 and retinoic acid receptors (RARs and RXRbeta) genes. Holtzman male rats from control and vitamin-A-deficient groups were maintained under 12-h-light:12-h-dark or 12-h-dark:12-h-dark conditions during the last week of treatment. RARalpha, RARbeta, RXRbeta, BMAL1 and PER1 transcript and protein levels were determined in hippocampus samples isolated every 4 h in a 24-h period. Regulatory regions of RARs and RXRbeta genes were scanned for clock-responsive sites, while BMAL1 and PER1 promoters were analyzed for retinoic acid responsive elements and retinoid X responsive elements. E-box and retinoid-related orphan receptor responsive element sites were found on regulatory regions of retinoid receptors genes, which display an endogenously controlled circadian expression in the rat hippocampus. Those temporal profiles were modified when animals were fed with a vitamin-A-deficient diet. Similarly, the nutritional vitamin A deficiency phase shifted BMAL1 and abolished PER1 circadian expression at both mRNA and protein levels. Our data suggest that vitamin A deficiency may affect the circadian expression in the hippocampus by modifying the rhythmic profiles of retinoic acid receptors. CI - Copyright (c) 2013 Elsevier Inc. All rights reserved. FAU - Navigatore-Fonzo, Lorena S AU - Navigatore-Fonzo LS AD - Laboratory of Chronobiology, Multidisciplinary Institute of Biological Research San Luis (IMIBIO-SL), National Council of Science and Technology (CONICET), National University of San Luis (UNSL). Chacabuco y Pedernera, D5700HHW, San Luis, Argentina. FAU - Golini, Rebeca L AU - Golini RL FAU - Ponce, Ivana T AU - Ponce IT FAU - Delgado, Silvia M AU - Delgado SM FAU - Plateo-Pignatari, Maria G AU - Plateo-Pignatari MG FAU - Gimenez, Maria S AU - Gimenez MS FAU - Anzulovich, Ana C AU - Anzulovich AC LA - eng GR - R01 TW006974/TW/FIC NIH HHS/United States GR - R01-TW006974/TW/FIC NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20120816 PL - United States TA - J Nutr Biochem JT - The Journal of nutritional biochemistry JID - 9010081 RN - 0 (ARNTL Transcription Factors) RN - 0 (PER1 protein, human) RN - 0 (Period Circadian Proteins) RN - 0 (RARA protein, human) RN - 0 (RNA, Messenger) RN - 0 (Rara protein, rat) RN - 0 (Receptors, Retinoic Acid) RN - 0 (Retinoic Acid Receptor alpha) RN - 0 (Retinoid X Receptor beta) RN - 0 (retinoic acid receptor beta) SB - IM MH - ARNTL Transcription Factors/genetics/metabolism MH - Animals MH - Circadian Rhythm/*physiology MH - *Diet MH - Gene Expression Regulation MH - Hippocampus/*metabolism MH - Male MH - Period Circadian Proteins/genetics/metabolism MH - Polymerase Chain Reaction MH - RNA, Messenger/genetics/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Receptors, Retinoic Acid/genetics/*metabolism MH - Retinoic Acid Receptor alpha MH - Retinoid X Receptor beta/genetics/*metabolism MH - Vitamin A Deficiency/*metabolism PMC - PMC3504648 MID - NIHMS384703 EDAT- 2012/08/21 06:00 MHDA- 2013/12/16 06:00 PMCR- 2014/05/01 CRDT- 2012/08/21 06:00 PHST- 2012/01/16 00:00 [received] PHST- 2012/04/27 00:00 [revised] PHST- 2012/05/07 00:00 [accepted] PHST- 2012/08/21 06:00 [entrez] PHST- 2012/08/21 06:00 [pubmed] PHST- 2013/12/16 06:00 [medline] PHST- 2014/05/01 00:00 [pmc-release] AID - S0955-2863(12)00148-9 [pii] AID - 10.1016/j.jnutbio.2012.05.006 [doi] PST - ppublish SO - J Nutr Biochem. 2013 May;24(5):859-67. doi: 10.1016/j.jnutbio.2012.05.006. Epub 2012 Aug 16.