PMID- 22905612
OWN - NLM
STAT- MEDLINE
DCOM- 20150515
LR  - 20120821
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 26
IP  - 7
DP  - 2012 Jul
TI  - [Transplantation of transforming growth factor beta3 gene-modified nucleus 
      pulposus cells for intervertebral disc degeneration in rabbits].
PG  - 790-5
AB  - OBJECTIVE: To evaluate the cell biological features and the effect of 
      transplantation of transforming growth factor beta3 (TGF-beta3) gene-modified 
      nucleus pulposus (NP) cells on the degeneration of lumbar intervertebral discs in 
      vitro. METHODS: NP cells at passage 2 were infected by recombinant adenovirus 
      carrying TGF-beta3 (Ad-TGF-beta3) gene (Ad-TGF-beta3 group), and then the cell 
      biological features were observed by cell vitality assay, the expression of the 
      TGF-beta3 protein was determined by Western blot, the expression of collagen type 
      II in logarithmic growth phase was determined by immunocytochemistry. The cells 
      with adenovirus-transfected (Adv group) and the un-transfected cells (blank 
      group) were used as controls. The model of lumbar disc degeneration was 
      established by needling L(3, 4), L(4, 5), and L(5, 6) in 30 New Zealand rabbits 
      (weighing 3.2-3.5 kg, male or female). Then Ad-TGF-beta3-transfected rabbit 
      degenerative nucleus pulposus cells (100 microL, 1 x 10(5)/mL, group A, n=12), no 
      gene-modified nucleus pulposus cells (100 microL, 1 x 10(5)/mL, group B, n=12), 
      and phosphate-buffered saline (PBS, 100 microL, group C, n=6) were injected into 
      degenerative lumbar intervertebral discs, respectively. L(3, 4), L(4, 5), and 
      L(5, 6) disc were harvested from the rabbits (4 in groups A and B, 2 in group C) 
      at 6, 10, and 14 weeks respectively to perform histological observation and 
      detect the expression of collagen type II and proteoglycan by RT-PCR. RESULTS: 
      The viability of nucleus pulposus cells was obviously improved after transfected 
      by recombinant Ad-TGF-beta3 gene. At 3, 7, and 14 days after transfected, 
      TGF-beta3 expression gradually increased in nucleus pulposus cells. The positive 
      staining of collagen type II was seen in Ad-TGF-beta3 group, and the positive 
      rate was significantly higher than that of Adv group and blank group (P < 0.05). 
      The disc degeneration in group A was slighter than that in groups B and C. The 
      expressions of collagen type II mRNA and proteoglycan mRNA in group A were 
      significantly higher than those in groups B and C at 6, 10, and 14 weeks (P < 
      0.05). CONCLUSION: TGF-beta3 can improve the biological activity of NP cells and 
      promote the biosynthesis of collagen type II and proteoglycan in intervertebral 
      discs, alleviate the degeneration of intervertebral discs after transplantation.
FAU - Song, Xiyuan
AU  - Song X
AD  - School of Basic Medicine, lining Medical University, lining Shandong, 272067, PR 
      China. sxy0726354@163.com
FAU - Peng, Shengzhi
AU  - Peng S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Collagen Type II)
RN  - 0 (Proteoglycans)
RN  - 0 (Transforming Growth Factor beta3)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Cell Transplantation/*methods
MH  - Cells, Cultured
MH  - Collagen Type II/genetics/metabolism
MH  - Female
MH  - Intervertebral Disc/*drug effects/*metabolism
MH  - Intervertebral Disc Degeneration/metabolism/*therapy
MH  - Male
MH  - Proteoglycans/*metabolism
MH  - Rabbits
MH  - Transfection
MH  - Transforming Growth Factor beta3/genetics/metabolism/*pharmacology
EDAT- 2012/08/22 06:00
MHDA- 2015/05/16 06:00
CRDT- 2012/08/22 06:00
PHST- 2012/08/22 06:00 [entrez]
PHST- 2012/08/22 06:00 [pubmed]
PHST- 2015/05/16 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 Jul;26(7):790-5.