PMID- 22906091
OWN - NLM
STAT- MEDLINE
DCOM- 20140203
LR  - 20181202
IS  - 1365-2591 (Electronic)
IS  - 0143-2885 (Linking)
VI  - 46
IP  - 4
DP  - 2013 Apr
TI  - Characterization of a programmed necrosis process in 3-dimensional cultures of 
      dental pulp fibroblasts.
PG  - 308-16
LID - 10.1111/j.1365-2591.2012.02114.x [doi]
AB  - AIM: To analyse and compare the expression of necrosis markers in human lung and 
      dental pulp fibroblasts and to determine whether this process differs by the type 
      of mesenchymal cell. METHODS: Human dental pulp fibroblasts were obtained from 
      unerupted third molars. Sound lung and pulpal fibroblasts were cultured in vitro 
      as spheroids to determine the expression of the necrosis hallmark 
      cyclooxygenase-2 (COX-2) mRNA using RT-PCR and the concentrations of vascular 
      endothelial growth factor (VEGF) and hepatocyte growth factor/scatter factor 
      (HGF/SF) proteins using an ELISA test. Cell viability within spheroids was also 
      compared with spheroid diameters over time. RESULTS: Increased expression of 
      COX-2 and VEGF was found in all spheroids compared with corresponding monolayers. 
      Although HGF/SF was highly expressed in MRC5 cells, dental pulp fibroblasts 
      aggregates maintained only a basal level compared with monolayer cultures. 
      Further, the observed progressive loss of viable cells explained the decreased 
      diameters of spheroids over time. The results demonstrate that necrosis occurs in 
      sound lung and pulpal fibroblasts. This cell death also displays differences 
      between these two different cell types, as they do not produce the same growth 
      factors quantity release. CONCLUSIONS: The necrosis process occurred in human 
      dental pulp fibroblasts and is different between the two cell types studied. This 
      in vitro experimental necrosis model could become an interesting inflammatory 
      tool. More investigations are needed to compare necrosis process in dental pulp 
      fibroblast and inflammation during pulpitis.
CI  - (c) 2012 International Endodontic Journal.
FAU - Le Clerc, J
AU  - Le Clerc J
AD  - Faculte d'Odontologie, Laboratoire de Biomateriaux en Site Osseux, UMR CNRS 6226, 
      Sciences Chimiques de Rennes, Universite de Rennes 1, Rennes, France.
FAU - Perard, M
AU  - Perard M
FAU - Pellen-Mussi, P
AU  - Pellen-Mussi P
FAU - Novella, A
AU  - Novella A
FAU - Tricot-Doleux, S
AU  - Tricot-Doleux S
FAU - Jeanne, S
AU  - Jeanne S
FAU - Perez, F
AU  - Perez F
LA  - eng
PT  - Journal Article
DEP - 20120821
PL  - England
TA  - Int Endod J
JT  - International endodontic journal
JID - 8004996
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
MH  - Cell Survival
MH  - Cyclooxygenase 2/biosynthesis
MH  - Dental Pulp/*cytology/metabolism
MH  - Dental Pulp Necrosis/*pathology
MH  - Fibroblasts/*metabolism
MH  - Hepatocyte Growth Factor/biosynthesis
MH  - Humans
MH  - Mesenchymal Stem Cells/metabolism
MH  - Necrosis/metabolism
MH  - Spheroids, Cellular/*metabolism
MH  - Vascular Endothelial Growth Factor A/biosynthesis
EDAT- 2012/08/22 06:00
MHDA- 2014/02/04 06:00
CRDT- 2012/08/22 06:00
PHST- 2012/01/24 00:00 [received]
PHST- 2012/07/07 00:00 [accepted]
PHST- 2012/08/22 06:00 [entrez]
PHST- 2012/08/22 06:00 [pubmed]
PHST- 2014/02/04 06:00 [medline]
AID - 10.1111/j.1365-2591.2012.02114.x [doi]
PST - ppublish
SO  - Int Endod J. 2013 Apr;46(4):308-16. doi: 10.1111/j.1365-2591.2012.02114.x. Epub 
      2012 Aug 21.