PMID- 22906152
OWN - NLM
STAT- MEDLINE
DCOM- 20130524
LR  - 20121128
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 37
IP  - 12
DP  - 2012 Dec
TI  - Intravitreal injection of soluble erythropoietin receptor exacerbates 
      photoreceptor cell apoptosis in a rat model of retinal detachment.
PG  - 1156-64
LID - 10.3109/02713683.2012.713156 [doi]
AB  - PURPOSE: To evaluate the effects of intravitreal injection of soluble 
      erythropoietin (EPO) receptor (sEPOR) on photoreceptor cell apoptosis in an 
      animal model of retinal detachment (RD). METHODS: Various dosages of sEPOR (2, 
      20, or 200 ng) were injected into the vitreous cavities of normal rats. Three 
      days after injection, retinal function was measured by flash electroretinography 
      (ERG). On day 7, histopathology and retinal morphology were examined by light and 
      transmission electron microscopy (TEM), respectively. Rat models of RD were 
      successfully established by injection of 1.4% sodium hyaluronate into the 
      subretinal space, followed by immediate injection of phosphate-buffered saline 
      (PBS) or sEPOR into the vitreous cavity. On day 3, photoreceptor cell apoptosis 
      was evaluated using terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL), and caspase-3 activity assayed by Western blotting and 
      immunofluorescence. Light microscopic examination of retinal histopathology was 
      used to determine the thickness of the outer nuclear layer (ONL) 14 days after 
      establishment of RD. RESULTS: There were no significant differences in the 
      latency and amplitude of maximal a, b and oscillatory potential (OP) wave 
      responses by flash ERG before or 3 days after sEPOR injection (p > 0.05). Retinal 
      tissues showed no obvious pathological changes by either light or transmission 
      electron microscopy. Both Western blotting and immunofluorescence indicated 
      consistent sEPOR enhanced caspase-3 activation aggravated apoptosis of 
      photoreceptor cells in RD rat retinas. On day 14, RD ONLs were thinner, according 
      to increasing dosages of sEPOR. CONCLUSION: Intravitreal injection of sEPOR 
      exacerbates photoreceptor cell apoptosis in RD models via activation of 
      caspase-3.
FAU - Chen, Fang
AU  - Chen F
AD  - Department of Ophthalmology, Clinical Medicine School, Yangzhou University, Subei 
      People's Hospital of Jiangsu Province, No. 98 Nantong West Road, Yangzhou, 
      Jiangsu, China.
FAU - Xie, Zhenggao
AU  - Xie Z
FAU - Wu, Xingwei
AU  - Wu X
FAU - Du, Wei
AU  - Du W
FAU - Wang, Jian
AU  - Wang J
FAU - Zhu, Jun
AU  - Zhu J
FAU - Ji, Heqing
AU  - Ji H
FAU - Wang, Yakun
AU  - Wang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120820
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Receptors, Erythropoietin)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Caspase 3/metabolism
MH  - *Disease Models, Animal
MH  - Electroretinography
MH  - Fluorescent Antibody Technique, Indirect
MH  - In Situ Nick-End Labeling
MH  - Intravitreal Injections
MH  - Male
MH  - Photic Stimulation
MH  - Photoreceptor Cells, Vertebrate/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Erythropoietin/*administration & dosage
MH  - Retinal Detachment/enzymology/*pathology
EDAT- 2012/08/22 06:00
MHDA- 2013/05/28 06:00
CRDT- 2012/08/22 06:00
PHST- 2012/08/22 06:00 [entrez]
PHST- 2012/08/22 06:00 [pubmed]
PHST- 2013/05/28 06:00 [medline]
AID - 10.3109/02713683.2012.713156 [doi]
PST - ppublish
SO  - Curr Eye Res. 2012 Dec;37(12):1156-64. doi: 10.3109/02713683.2012.713156. Epub 
      2012 Aug 20.