PMID- 22906174
OWN - NLM
STAT- MEDLINE
DCOM- 20130116
LR  - 20120821
IS  - 1091-7691 (Electronic)
IS  - 0895-8378 (Linking)
VI  - 24
IP  - 10
DP  - 2012 Aug
TI  - Changed gene expression in brains of mice exposed to traffic in a highway tunnel.
PG  - 676-86
LID - 10.3109/08958378.2012.714004 [doi]
AB  - CONTEXT: Air pollution has been suggested to have an impact on the brain. 
      OBJECTIVE: The objective was to assess the expression of inflammation-related 
      genes in the brains of mice that had been exposed for 5 days to a 
      well-characterized traffic-polluted environment, i.e. a highway tunnel. MATERIALS 
      AND METHODS: Twenty C57BL6 mice were randomly allocated to four groups of five 
      animals. Two groups were placed in the tunnel for 5 days (mean PM 2.5, 55.1 
      mug/m(3), mean elemental carbon, EC 13.9 mug/m(3)) in cages with or without filter, two 
      control groups were housed outside the tunnel. Animals were assessed within 24 
      hours after the last exposure day. Lung injury and inflammation were assessed by 
      bronchoalveolar lavage (BAL) and histology. Blood leukocytosis and coagulation 
      parameters were determined in peripheral blood. The olfactory bulb and 
      hippocampus were analyzed for changes in expression of inflammatory genes and 
      brain-derived neurotrophic factor (BDNF). RESULTS AND DISCUSSION: Although carbon 
      particles were abundant in alveolar macrophages of exposed mice and absent in 
      non-exposed mice, there was no evidence of pulmonary or systemic inflammation. 
      There was an increased expression of genes involved in inflammatory response 
      (COX2, NOS2, NOS3, and NFE2L2) in the hippocampus of the exposed mice. In the 
      olfactory bulb, a downregulation was found for IL1alpha, COX2, NFE2L2, IL6, and BDNF. 
      CONCLUSION: Although this short-term exposure to traffic-related pollution did 
      not induce pulmonary or systemic inflammation, the expression of inflammatory 
      genes was affected in different brain areas. The decreased BDNF expression in the 
      olfactory bulb suggests lower brain neurotrophic support in response to 
      traffic-related air pollution.
FAU - Bos, Inge
AU  - Bos I
AD  - Flemish Institute for Technological Research (VITO), Mol, Belgium.
FAU - De Boever, Patrick
AU  - De Boever P
FAU - Emmerechts, Jan
AU  - Emmerechts J
FAU - Buekers, Jurgen
AU  - Buekers J
FAU - Vanoirbeek, Jeroen
AU  - Vanoirbeek J
FAU - Meeusen, Romain
AU  - Meeusen R
FAU - Van Poppel, Martine
AU  - Van Poppel M
FAU - Nemery, Benoit
AU  - Nemery B
FAU - Nawrot, Tim
AU  - Nawrot T
FAU - Panis, Luc Int
AU  - Panis LI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
RN  - 0 (Air Pollutants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vehicle Emissions)
SB  - IM
MH  - Air Pollutants/pharmacokinetics/*toxicity
MH  - Animals
MH  - Belgium
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Hippocampus/*drug effects/immunology/metabolism
MH  - Inhalation Exposure/*adverse effects
MH  - Lung/drug effects/immunology/metabolism/pathology
MH  - Macrophages, Alveolar/drug effects/immunology/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-E2-Related Factor 2/genetics/metabolism
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neurons/drug effects/immunology/metabolism
MH  - Olfactory Bulb/*drug effects/immunology/metabolism
MH  - Organ Specificity
MH  - RNA, Messenger/metabolism
MH  - Random Allocation
MH  - Tissue Distribution
MH  - Urban Health
MH  - Vehicle Emissions/analysis/*toxicity
EDAT- 2012/08/22 06:00
MHDA- 2013/01/17 06:00
CRDT- 2012/08/22 06:00
PHST- 2012/08/22 06:00 [entrez]
PHST- 2012/08/22 06:00 [pubmed]
PHST- 2013/01/17 06:00 [medline]
AID - 10.3109/08958378.2012.714004 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2012 Aug;24(10):676-86. doi: 10.3109/08958378.2012.714004.