PMID- 22906961
OWN - NLM
STAT- MEDLINE
DCOM- 20130116
LR  - 20211021
IS  - 1554-8635 (Electronic)
IS  - 1554-8627 (Print)
IS  - 1554-8627 (Linking)
VI  - 8
IP  - 9
DP  - 2012 Sep
TI  - A short linear motif in BNIP3L (NIX) mediates mitochondrial clearance in 
      reticulocytes.
PG  - 1325-32
LID - 10.4161/auto.20764 [doi]
AB  - Elimination of defective mitochondria is essential for the health of long-lived, 
      postmitotic cells. To gain insight into this process, we examined programmed 
      mitochondrial clearance in reticulocytes. BNIP3L is a mitochondrial outer 
      membrane protein that is required for clearance. It has been suggested that 
      BNIP3L functions by causing mitochondrial depolarization, activating autophagy, 
      or engaging the autophagy machinery. Here we showed in mice that BNIP3L activity 
      localizes to a small region in its cytoplasmic domain, the minimal essential 
      region (MER). The MER is a novel sequence, which comprises three contiguous 
      hydrophobic amino acid residues, and flanking charged residues. Mutation of the 
      central leucine residue causes complete loss of BNIP3L activity, and prevents 
      rescue of mitochondrial clearance. Structural bioinformatics analysis predicts 
      that the BNIP3L cytoplasmic domain lacks stable tertiary structure, but that the 
      MER forms an alpha-helix upon binding to another protein. These findings support an 
      adaptor model of BNIP3L, centered on the MER.
FAU - Zhang, Ji
AU  - Zhang J
AD  - Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, TN, 
      USA.
FAU - Loyd, Melanie R
AU  - Loyd MR
FAU - Randall, Mindy S
AU  - Randall MS
FAU - Waddell, M Brett
AU  - Waddell MB
FAU - Kriwacki, Richard W
AU  - Kriwacki RW
FAU - Ney, Paul A
AU  - Ney PA
LA  - eng
GR  - R21 DK074519/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120821
PL  - United States
TA  - Autophagy
JT  - Autophagy
JID - 101265188
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Nix protein, mouse)
RN  - 0 (bcl-X Protein)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - Computational Biology
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Leucine/metabolism
MH  - Membrane Proteins/*chemistry/deficiency/*metabolism
MH  - Mice
MH  - Mitochondria/*metabolism
MH  - Mitochondrial Proteins/*chemistry/deficiency/*metabolism
MH  - Molecular Sequence Data
MH  - Protein Structure, Tertiary
MH  - Reticulocytes/*metabolism
MH  - Structure-Activity Relationship
MH  - bcl-X Protein/metabolism
PMC - PMC3442879
EDAT- 2012/08/22 06:00
MHDA- 2013/01/17 06:00
PMCR- 2013/09/01
CRDT- 2012/08/22 06:00
PHST- 2012/08/22 06:00 [entrez]
PHST- 2012/08/22 06:00 [pubmed]
PHST- 2013/01/17 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - 20764 [pii]
AID - 2012AUTO0203 [pii]
AID - 10.4161/auto.20764 [doi]
PST - ppublish
SO  - Autophagy. 2012 Sep;8(9):1325-32. doi: 10.4161/auto.20764. Epub 2012 Aug 21.