PMID- 22909062
OWN - NLM
STAT- MEDLINE
DCOM- 20130123
LR  - 20211021
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 9
DP  - 2012 Aug 21
TI  - Induction of interleukin-10 is dependent on p38 mitogen-activated protein kinase 
      pathway in macrophages infected with porcine reproductive and respiratory 
      syndrome virus.
PG  - 165
LID - 10.1186/1743-422X-9-165 [doi]
AB  - BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) causes 
      reproductive failure and respiratory illness in pigs and usually establishes a 
      persistent infection. Previous studies suggested that interleukin-10 (IL-10) 
      could play a critical role in PRRSV-induced immunosuppression. However, the 
      ability of PRRSV to induce IL-10 in infected cells is controversial. In this 
      study, we further investigated this issue using PRRSV strain CH-1a, which is the 
      first North American genotype strain isolated in China. RESULTS: PRRSV strain 
      CH-1a could significantly up-regulate IL-10 production both at mRNA and protein 
      levels in porcine alveolar macrophages (PAMs), bone marrow-derived macrophages 
      (BMDMs), and monocyte-derived macrophages (MDMs). However, up-regulation of IL-10 
      by PRRSV was retarded by specific inhibitors of p38 mitogen-activated protein 
      kinase (MAPK) (SB203580) and NF-kappaB (BAY11-7082). Additionally, p38 MAPK and NF-kappaB 
      pathways but not ERK1/2 MAPK were actually activated in PRRSV-infected BMDMs as 
      demonstrated by western blot analysis, suggesting that p38 MAPK and NF-kappaB 
      pathways are involved in the induction of IL-10 by PRRSV infection. Transfection 
      of PAMs and PAM cell line 3D4/21 (CRL-2843) with viral structural genes showed 
      that glycoprotein5 (GP5) could significantly up-regulate IL-10 production, which 
      was dependent on p38 MAPK and signal transducer and activator of transcription-3 
      (STAT3) activation. We also demonstrated that a full-length glycoprotein was 
      essential for GP5 to induce IL-10 production. CONCLUSIONS: PRRSV strain CH-1a 
      could significantly up-regulate IL-10 production through p38 MAPK activation.
FAU - Hou, Jun
AU  - Hou J
AD  - State Key Laboratories of Agrobiotechnology, Department of Microbiology and 
      Immunology, College of Biological Science, China Agricultural University, 
      Beijing, 100193, China.
FAU - Wang, Lianghai
AU  - Wang L
FAU - Quan, Rong
AU  - Quan R
FAU - Fu, Yi
AU  - Fu Y
FAU - Zhang, Hexiao
AU  - Zhang H
FAU - Feng, Wen-hai
AU  - Feng WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120821
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Line
MH  - China
MH  - Gene Expression Profiling
MH  - Interleukin-10/*biosynthesis/immunology
MH  - Macrophages/*immunology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - p38 Mitogen-Activated Protein Kinases/immunology/*metabolism
PMC - PMC3441385
EDAT- 2012/08/23 06:00
MHDA- 2013/01/24 06:00
PMCR- 2012/08/21
CRDT- 2012/08/23 06:00
PHST- 2012/01/26 00:00 [received]
PHST- 2012/08/07 00:00 [accepted]
PHST- 2012/08/23 06:00 [entrez]
PHST- 2012/08/23 06:00 [pubmed]
PHST- 2013/01/24 06:00 [medline]
PHST- 2012/08/21 00:00 [pmc-release]
AID - 1743-422X-9-165 [pii]
AID - 10.1186/1743-422X-9-165 [doi]
PST - epublish
SO  - Virol J. 2012 Aug 21;9:165. doi: 10.1186/1743-422X-9-165.