PMID- 22909461
OWN - NLM
STAT- MEDLINE
DCOM- 20130321
LR  - 20200319
IS  - 1743-1328 (Electronic)
IS  - 0161-6412 (Linking)
VI  - 34
IP  - 9
DP  - 2012 Nov
TI  - Phosphorylation of Akt is involved in protocatechuic acid-induced neurotrophic 
      activity.
PG  - 901-7
LID - 10.1179/1743132812Y.0000000086 [doi]
AB  - OBJECTIVE: To investigate the mechanisms underlying protocatechuic acid 
      (PCA)-induced neurotrophic effects on cultured cortical neurons. METHODS: The 
      mRNA expression of microtubule-associated protein 2 (MAP2) and brain-derived 
      neurotrophic factor (BDNF) were measured by real-time quantitative PCR (qPCR). 
      Subsequently, antagonists were used to study the signaling pathways activated by 
      PCA and western blotting was used to detect the phosphorylation level of 
      kinase-related protein. RESULTS: The mRNA expression of MAP2 and BDNF were 
      upregulated in neurons treated with PCA compared with vehicle control. 
      PCA-induced neurite outgrowth and neuronal survival in cultured cortical neurons 
      were significantly inhibited by ZM241385 (an A(2A) receptor antagonist) and 
      LY294002 (a PI3K inhibitor), but not by K252a (a TrkA receptor antagonist), 
      GO6976 (a protein kinase C inhibitor) and PD98059 (a MEK inhibitor). PCA enhanced 
      the phosphorylation of Akt, which could be blocked by LY294002. CONCLUSION: The 
      PI3K/Akt signaling pathway might play an important role in the neurotrophic 
      activity of PCA.
FAU - Xue, Xiao-Yan
AU  - Xue XY
AD  - Ganzhou People's Hospital, Ganzhou, China.
FAU - Liao, Min-Jing
AU  - Liao MJ
FAU - Lin, Lian-Feng
AU  - Lin LF
FAU - Zhang, Zheng
AU  - Zhang Z
FAU - Zhou, Xiao-Wen
AU  - Zhou XW
FAU - Zhou, Xing
AU  - Zhou X
FAU - Luo, Huan-Min
AU  - Luo HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120820
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hydroxybenzoates)
RN  - 0 (MAP2 protein, rat)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (RNA, Messenger)
RN  - 36R5QJ8L4B (protocatechuic acid)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Anticarcinogenic Agents/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Hydroxybenzoates/*pharmacology
MH  - Microtubule-Associated Proteins/genetics
MH  - Neurons/*drug effects
MH  - Oncogene Protein v-akt/*metabolism
MH  - Phosphorylation/drug effects
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
EDAT- 2012/08/23 06:00
MHDA- 2013/03/22 06:00
CRDT- 2012/08/23 06:00
PHST- 2012/08/23 06:00 [entrez]
PHST- 2012/08/23 06:00 [pubmed]
PHST- 2013/03/22 06:00 [medline]
AID - ner2731 [pii]
AID - 10.1179/1743132812Y.0000000086 [doi]
PST - ppublish
SO  - Neurol Res. 2012 Nov;34(9):901-7. doi: 10.1179/1743132812Y.0000000086. Epub 2012 
      Aug 20.