PMID- 22910180
OWN - NLM
STAT- MEDLINE
DCOM- 20121210
LR  - 20191210
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 91
IP  - 11-12
DP  - 2012 Oct 5
TI  - Anti-inflammatory and immunomodulatory effects of iridoid glycosides from 
      Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats.
PG  - 369-376
LID - S0024-3205(12)00426-2 [pii]
LID - 10.1016/j.lfs.2012.08.013 [doi]
AB  - AIMS: Uric acid nephropathy (UAN) is due to excessive uric acid, which leads to 
      hyperuricemia and kidney damage via the deposition of urate microcrystals in the 
      kidneys. Iridoid glycosides of Paederia scandens (IGPS) is a major active 
      component isolated from the traditional Chinese herb P. scandens (LOUR.) MERRILL 
      (Rubiaceae). This study aimed to evaluate the anti-inflammatory and 
      immunomodulatory effects of IGPS and its mechanism on UAN rats. MAIN METHODS: The 
      experimental model of UAN rats was induced by using uricopoiesis promoter adenine 
      and uricase inhibitor potassium oxonate (PO). Treatment groups received three 
      different doses of IGPS, allopurinol (AP) and benzbromarone (BEN) daily for 
      24days respectively. The histopathology of renal tissues in UAN rats were 
      assessed for conventional morphological evaluation. The nuclear factor-kappaBp65 
      (NF-kappaBp65), monocyte chemoattractant protein-1 (MCP-1) and alpha-smooth muscle actin 
      (alpha-SMA) protein expression of renal tissues in UAN rats were investigated by 
      immunohistochemistry. MCP-1 and alpha-SMA mRNA levels were monitored by method of 
      reverse transcription polymerase chain reaction (RT-PCR). KEY FINDINGS: Treatment 
      with IGPS significantly ameliorated UAN induced renal tissue injury, inhibited 
      the biological activity of NF-kappaBp65, MCP-1 and alpha-SMA, and suppressed the mRNA 
      expressions of MCP-1 and alpha-SMA. SIGNIFICANCE: IGPS exerts a protective effect 
      against renal injury in UAN rats, possesses anti-inflammatory and 
      immunomodulatory effects by inactivating NF-kappaBp65 pathway transmembrane signal 
      transduction, down regulating the expression of MCP-1 and alpha-SMA to modulate 
      pro-inflammatory mediator production in nephropathy tissue to improve renal 
      fibrosis in UAN rats.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Zhu, Wenjing
AU  - Zhu W
AD  - Department of Pharmacology, Anhui Medical University, Hefei, Anhui, China.
FAU - Pang, Mingqun
AU  - Pang M
AD  - Department of Pharmacology, Anhui Medical University, Hefei, Anhui, China.
FAU - Dong, Liuyi
AU  - Dong L
AD  - Department of Pharmacology, Anhui Medical University, Hefei, Anhui, China.
FAU - Huang, Xueying
AU  - Huang X
AD  - Department of Anatomy, Anhui Medical University, Hefei, Anhui, China.
FAU - Wang, Shuangmiao
AU  - Wang S
AD  - Department of Pharmacology, Anhui Medical University, Hefei, Anhui, China.
FAU - Zhou, Lanlan
AU  - Zhou L
AD  - Department of Pharmacology, Anhui Medical University, Hefei, Anhui, China. 
      Electronic address: lanlzhou@yahoo.com.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120815
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Actins)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cyclopentane Monoterpenes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Glucosides)
RN  - 0 (Immunologic Factors)
RN  - 0 (Iridoid Glycosides)
RN  - 0 (Pyrans)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (smooth muscle actin, rat)
RN  - 268B43MJ25 (Uric Acid)
RN  - 4POG0RL69O (Benzbromarone)
RN  - 63CZ7GJN5I (Allopurinol)
RN  - V3CFI02X39 (asperuloside)
SB  - IM
MH  - Actins/analysis
MH  - Allopurinol/therapeutic use
MH  - Animals
MH  - Anti-Inflammatory Agents/*therapeutic use
MH  - Benzbromarone/therapeutic use
MH  - Chemokine CCL2/analysis
MH  - Cyclopentane Monoterpenes
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Glucosides/therapeutic use
MH  - Immunologic Factors/*therapeutic use
MH  - Iridoid Glycosides/*therapeutic use
MH  - Kidney/chemistry/drug effects/pathology
MH  - Kidney Diseases/blood/*drug therapy/etiology/pathology
MH  - Male
MH  - Medicine, Chinese Traditional
MH  - Pyrans/therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Rubiaceae
MH  - Transcription Factor RelA/analysis
MH  - Uric Acid/*blood
EDAT- 2012/08/23 06:00
MHDA- 2012/12/12 06:00
CRDT- 2012/08/23 06:00
PHST- 2012/04/03 00:00 [received]
PHST- 2012/07/22 00:00 [revised]
PHST- 2012/08/02 00:00 [accepted]
PHST- 2012/08/23 06:00 [entrez]
PHST- 2012/08/23 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - S0024-3205(12)00426-2 [pii]
AID - 10.1016/j.lfs.2012.08.013 [doi]
PST - ppublish
SO  - Life Sci. 2012 Oct 5;91(11-12):369-376. doi: 10.1016/j.lfs.2012.08.013. Epub 2012 
      Aug 15.