PMID- 229111
OWN - NLM
STAT- MEDLINE
DCOM- 19800226
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 83
IP  - 1
DP  - 1979 Oct
TI  - Restriction of patching of bound concanavalin A after incorporation of 
      arachidonic acid into the plasma membrane of virally transformed fibroblasts.
PG  - 218-30
AB  - Topographical distribution of concanavalin A binding sites (CABS) was studied in 
      two lines of virally transformed fibroblasts as a function of fatty acid 
      composition. Fatty acid composition was manipulated by incubating cells in fatty 
      acid, ATP, CoA, and delipidated fetal calf serum (FCS). VLM cells grown in medium 
      containing 5% FCS have a clustered CABS distribution. Plasma membrane vesicles 
      (PMVs) derived from these cells have an arachidonate content of 1.7%. Elevation 
      of PMV arachidonate to 15.8% results in a marked restriction of CABS patching, 
      while elevation to 6.8% is associated with intermediate restriction of patching. 
      Restriction of patching is associated with increased microviscosity. CABS of Rous 
      sarcoma virus-transformed chicken embryo fibroblasts (RSV-CEF) are also 
      responsive to arachidonate enrichment medium. Whereas untreated cells have a 
      clustered CABS distribution, cells incubated for 24 h in arachidonate enrichment 
      medium have predominantly a dispersed CABS distribution. In both VLM cells and 
      RSV-CEF, ATP, CoA, and delipidated FCS alone have no effect upon CABS mobility. 
      Inhibition of CABS patching is also observed when aspirin is included in the 
      arachidonate enrichment medium but not when the cells are incubated in 
      prostaglandins, thus suggesting that the restriction of CABS mobility is not 
      mediated by prostaglandins. Other fatty acids (palmitate, oleate, nonadecanoate) 
      failed to restrict CABS movement. The inhibition of CABS mobility is independent 
      of cell shape change.
FAU - Hill, D J
AU  - Hill DJ
FAU - Borysenko, J Z
AU  - Borysenko JZ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Arachidonic Acids)
RN  - 0 (Receptors, Concanavalin A)
RN  - 11028-71-0 (Concanavalin A)
SB  - IM
MH  - Animals
MH  - Arachidonic Acids/*metabolism
MH  - Cell Line
MH  - Cell Membrane/*metabolism
MH  - Cell Transformation, Viral
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Concanavalin A/metabolism
MH  - Embryo, Mammalian/physiology
MH  - Mice
MH  - Receptors, Concanavalin A/*metabolism
MH  - Simian virus 40
PMC - PMC2110438
EDAT- 1979/10/01 00:00
MHDA- 1979/10/01 00:01
PMCR- 1980/04/01
CRDT- 1979/10/01 00:00
PHST- 1979/10/01 00:00 [pubmed]
PHST- 1979/10/01 00:01 [medline]
PHST- 1979/10/01 00:00 [entrez]
PHST- 1980/04/01 00:00 [pmc-release]
AID - 80072246 [pii]
AID - 10.1083/jcb.83.1.218 [doi]
PST - ppublish
SO  - J Cell Biol. 1979 Oct;83(1):218-30. doi: 10.1083/jcb.83.1.218.