PMID- 22912838
OWN - NLM
STAT- MEDLINE
DCOM- 20130501
LR  - 20231105
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 8
DP  - 2012
TI  - Exercise does not protect against MPTP-induced neurotoxicity in BDNF 
      haploinsufficient mice.
PG  - e43250
LID - 10.1371/journal.pone.0043250 [doi]
LID - e43250
AB  - Exercise has been demonstrated to potently protect substantia nigra pars compacta 
      (SN) dopaminergic neurons from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine 
      (MPTP)-induced neurotoxicity. One mechanism proposed to account for this 
      neuroprotection is the upregulation of neurotrophic factors. Several neurotrophic 
      factors, including Brain Derived Neurotrophic Factor (BDNF), have been shown to 
      upregulate in response to exercise. In order to determine if exercise-induced 
      neuroprotection is dependent upon BDNF, we compared the neuroprotective effects 
      of voluntary exercise in mice heterozygous for the BDNF gene (BDNF+/-) with 
      strain-matched wild-type (WT) mice. Stereological estimates of SNpc DA neurons 
      from WT mice allowed 90 days exercise via unrestricted running demonstrated 
      complete protection against the MPTP-induced neurotoxicity. However, BDNF+/- mice 
      allowed 90 days of unrestricted exercise were not protected from MPTP-induced 
      SNpc DA neuron loss. Proteomic analysis comparing SN and striatum from 90 day 
      exercised WT and BDNF+/- mice showed differential expression of proteins related 
      to energy regulation, intracellular signaling and trafficking. These results 
      suggest that a full genetic complement of BDNF is critical for the 
      exercise-induced neuroprotection of SNpc DA neurons.
FAU - Gerecke, Kim M
AU  - Gerecke KM
AD  - Department of Psychology and Neuroscience Program, Rhodes College, Memphis, 
      Tennessee, United States of America. gereckek@rhodes.edu
FAU - Jiao, Yun
AU  - Jiao Y
FAU - Pagala, Viswajeeth
AU  - Pagala V
FAU - Smeyne, Richard J
AU  - Smeyne RJ
LA  - eng
GR  - R01 NS039006/NS/NINDS NIH HHS/United States
GR  - R01 NS070825/NS/NINDS NIH HHS/United States
GR  - NS039006/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120817
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - DNA Primers/genetics
MH  - Dopaminergic Neurons/drug effects/*physiology
MH  - Electrophoresis, Agar Gel
MH  - Genotype
MH  - *Haploinsufficiency
MH  - Immunohistochemistry
MH  - MPTP Poisoning/physiopathology/*prevention & control
MH  - Mice
MH  - Physical Conditioning, Animal/*physiology
MH  - Proteomics
MH  - Substantia Nigra/*cytology
MH  - Tandem Mass Spectrometry
PMC - PMC3422268
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/08/23 06:00
MHDA- 2013/05/02 06:00
PMCR- 2012/08/17
CRDT- 2012/08/23 06:00
PHST- 2012/04/18 00:00 [received]
PHST- 2012/07/18 00:00 [accepted]
PHST- 2012/08/23 06:00 [entrez]
PHST- 2012/08/23 06:00 [pubmed]
PHST- 2013/05/02 06:00 [medline]
PHST- 2012/08/17 00:00 [pmc-release]
AID - PONE-D-12-11031 [pii]
AID - 10.1371/journal.pone.0043250 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(8):e43250. doi: 10.1371/journal.pone.0043250. Epub 2012 Aug 17.