PMID- 22914941
OWN - NLM
STAT- MEDLINE
DCOM- 20130314
LR  - 20211021
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 916
DP  - 2012
TI  - Clonal and lineage analysis of melanocyte stem cells and their progeny in the 
      zebrafish.
PG  - 181-95
LID - 10.1007/978-1-61779-980-8_14 [doi]
AB  - The study of melanocyte biology in the zebrafish presents a highly tractable 
      system for understanding fundamental principles of developmental biology. 
      Melanocytes are visible in the transparent embryo and in the mature fish 
      following metamorphosis, a physical transformation from the larval to adult form. 
      While early developing larval melanocytes are direct derivatives of the neural 
      crest, the remainder of melanocytes develop from unpigmented precursors, or 
      melanocyte stem cells (MSCs). The Tol2 transposable element has facilitated the 
      construction of stable transgenic lines that label melanocytes. In another 
      application, integration of Tol2 constructs makes possible clonal analysis of 
      melanocyte and MSC lineages. Drugs that block melanin synthesis, ablate 
      melanocytes, and block establishment of MSC populations allow the interrogation 
      of this model system for mechanisms of adult stem cell development and 
      regulation.
FAU - Tryon, Robert C
AU  - Tryon RC
AD  - Department of Genetics, Washington School of Medicine, St. Louis, MO, USA.
FAU - Johnson, Stephen L
AU  - Johnson SL
LA  - eng
GR  - R01 GM056988/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Transposable Elements)
RN  - 6F82C6Q54C (Phenylthiourea)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - *Cell Differentiation/drug effects/radiation effects
MH  - Clone Cells/cytology/drug effects/metabolism/radiation effects
MH  - DNA Transposable Elements/genetics
MH  - Melanocytes/*cytology
MH  - Phenylthiourea/pharmacology
MH  - Staining and Labeling
MH  - Stem Cells/*cytology/drug effects/metabolism/radiation effects
MH  - X-Rays
MH  - Zebrafish/*embryology
PMC - PMC3630497
MID - NIHMS460578
EDAT- 2012/08/24 06:00
MHDA- 2013/03/15 06:00
PMCR- 2013/04/19
CRDT- 2012/08/24 06:00
PHST- 2012/08/24 06:00 [entrez]
PHST- 2012/08/24 06:00 [pubmed]
PHST- 2013/03/15 06:00 [medline]
PHST- 2013/04/19 00:00 [pmc-release]
AID - 10.1007/978-1-61779-980-8_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;916:181-95. doi: 10.1007/978-1-61779-980-8_14.