PMID- 22915589
OWN - NLM
STAT- MEDLINE
DCOM- 20121231
LR  - 20240512
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 287
IP  - 42
DP  - 2012 Oct 12
TI  - Anti-apoptotic role of caspase-cleaved GAB1 adaptor protein in hepatocyte growth 
      factor/scatter factor-MET receptor protein signaling.
PG  - 35382-35396
LID - S0021-9258(20)62685-0 [pii]
LID - 10.1074/jbc.M112.409797 [doi]
AB  - The GRB2-associated binder 1 (GAB1) docking/scaffold protein is a key mediator of 
      the MET-tyrosine kinase receptor activated by hepatocyte growth factor/scatter 
      factor (HGF/SF). Activated MET promotes recruitment and tyrosine phosphorylation 
      of GAB1, which in turn recruits multiple proteins and mediates MET signaling 
      leading to cell survival, motility, and morphogenesis. We previously reported 
      that, without its ligand, MET is a functional caspase target during apoptosis, 
      allowing the generation of a p40-MET fragment that amplifies apoptosis. In this 
      study we established that GAB1 is also a functional caspase target by evidencing 
      a caspase-cleaved p35-GAB1 fragment that contains the MET binding domain. GAB1 is 
      cleaved by caspases before MET, and the resulting p35-GAB1 fragment is 
      phosphorylated by MET upon HGF/SF binding and can interact with a subset of GAB1 
      partners, PI3K, and GRB2 but not with SHP2. This p35-GAB1 fragment favors cell 
      survival by maintaining HGF/SF-induced MET activation of AKT and by hindering 
      p40-MET pro-apoptotic function. These data demonstrate an anti-apoptotic role of 
      caspase-cleaved GAB1 in HGF/SF-MET signaling.
FAU - Le Goff, Arnaud
AU  - Le Goff A
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France.
FAU - Ji, Zongling
AU  - Ji Z
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France; Faculty of Life Sciences, C2222 
      Michael Smith Building, University of Manchester, Manchester, United Kingdom.
FAU - Leclercq, Berenice
AU  - Leclercq B
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France.
FAU - Bourette, Roland P
AU  - Bourette RP
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France.
FAU - Mougel, Alexandra
AU  - Mougel A
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France.
FAU - Guerardel, Cateline
AU  - Guerardel C
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France.
FAU - de Launoit, Yvan
AU  - de Launoit Y
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France.
FAU - Vicogne, Jerome
AU  - Vicogne J
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France.
FAU - Goormachtigh, Gautier
AU  - Goormachtigh G
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France.
FAU - Fafeur, Veronique
AU  - Fafeur V
AD  - CNRS UMR 8161, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, IFR142, Lille, France. Electronic address: 
      veronique.fafeur@ibl.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120822
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (GAB1 protein, human)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (GRB2 protein, human)
RN  - 0 (HGF protein, human)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (MET protein, human)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/*metabolism
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Caspases/genetics/*metabolism
MH  - Dogs
MH  - Enzyme Activation/physiology
MH  - GRB2 Adaptor Protein/genetics/metabolism
MH  - HeLa Cells
MH  - Hep G2 Cells
MH  - Hepatocyte Growth Factor/genetics/*metabolism
MH  - Humans
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - *Proteolysis
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - Proto-Oncogene Proteins c-met/genetics/*metabolism
MH  - Signal Transduction/*physiology
PMC - PMC3471683
EDAT- 2012/08/24 06:00
MHDA- 2013/01/01 06:00
PMCR- 2013/10/12
CRDT- 2012/08/24 06:00
PHST- 2012/08/24 06:00 [entrez]
PHST- 2012/08/24 06:00 [pubmed]
PHST- 2013/01/01 06:00 [medline]
PHST- 2013/10/12 00:00 [pmc-release]
AID - S0021-9258(20)62685-0 [pii]
AID - M112.409797 [pii]
AID - 10.1074/jbc.M112.409797 [doi]
PST - ppublish
SO  - J Biol Chem. 2012 Oct 12;287(42):35382-35396. doi: 10.1074/jbc.M112.409797. Epub 
      2012 Aug 22.