PMID- 22915817
OWN - NLM
STAT- MEDLINE
DCOM- 20130111
LR  - 20240316
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 22
DP  - 2012 Nov
TI  - Porcine circovirus type 2 induces autophagy via the AMPK/ERK/TSC2/mTOR signaling 
      pathway in PK-15 cells.
PG  - 12003-12
LID - 10.1128/JVI.01434-12 [doi]
AB  - Porcine circovirus type 2 (PCV2) uses autophagy machinery to enhance its 
      replication in PK-15 cells. However, the underlying mechanisms are unknown. By 
      the use of specific inhibitors, RNA interference, and coimmunoprecipitation, we 
      show that PCV2 induces autophagy in PK-15 cells through a pathway involving the 
      kinases AMP-activated protein kinase (AMPK) and extracellular signal-regulated 
      kinase 1/2 (ERK1/2), the tumor suppressor protein TSC2, and the mammalian target 
      of rapamycin (mTOR). AMPK and ERK1/2 positively regulate autophagy through 
      negative control of the mTOR pathway by phosphorylating TSC2 in PCV2-infected 
      PK-15 cells. Thus, PCV2 might induce autophagy via the AMPK/ERK/TSC2/mTOR 
      signaling pathway in the host cells, representing a pivotal mechanism for PCV2 
      pathogenesis.
FAU - Zhu, Binglin
AU  - Zhu B
AD  - Zhejiang University Institute of Preventive Veterinary Medicine, Zhejiang 
      Province Key Laboratory of Preventive Veterinary Medicine, Hangzhou, People's 
      Republic of China.
FAU - Zhou, Yingshan
AU  - Zhou Y
FAU - Xu, Fei
AU  - Xu F
FAU - Shuai, Jiangbing
AU  - Shuai J
FAU - Li, Xiaoliang
AU  - Li X
FAU - Fang, Weihuan
AU  - Fang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120822
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tuberous Sclerosis Complex 2 Protein)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Animals
MH  - *Autophagy
MH  - Cell Line
MH  - Circoviridae Infections/*metabolism
MH  - Circovirus/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Gene Expression Regulation, Viral
MH  - Models, Biological
MH  - Phosphorylation
MH  - RNA, Small Interfering/metabolism
MH  - Signal Transduction
MH  - Swine
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Tuberous Sclerosis Complex 2 Protein
MH  - Tumor Suppressor Proteins/*metabolism
PMC - PMC3486458
EDAT- 2012/08/24 06:00
MHDA- 2013/01/12 06:00
PMCR- 2013/05/01
CRDT- 2012/08/24 06:00
PHST- 2012/08/24 06:00 [entrez]
PHST- 2012/08/24 06:00 [pubmed]
PHST- 2013/01/12 06:00 [medline]
PHST- 2013/05/01 00:00 [pmc-release]
AID - JVI.01434-12 [pii]
AID - 01434-12 [pii]
AID - 10.1128/JVI.01434-12 [doi]
PST - ppublish
SO  - J Virol. 2012 Nov;86(22):12003-12. doi: 10.1128/JVI.01434-12. Epub 2012 Aug 22.