PMID- 22917596
OWN - NLM
STAT- MEDLINE
DCOM- 20130228
LR  - 20220317
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 905
DP  - 2012 Sep 15
TI  - Highly sensitive and quantitative profiling of acidic phytohormones using 
      derivatization approach coupled with nano-LC-ESI-Q-TOF-MS analysis.
PG  - 67-74
LID - 10.1016/j.jchromb.2012.08.005 [doi]
AB  - In current study, we developed a highly sensitive method for the quantitative 
      profiling of acidic phytohormones. Tandem solid-phase extraction (SPE) and 
      liquid-liquid extraction (LLE) was employed to efficiently purify acidic 
      phytohormones, which were further derived by 3-bromoactonyltrimethylammonium 
      bromide (BTA) to increase the ionization efficiency in electrospray 
      ionization-mass spectrometry detection. Additionally, fifteen BTA-derived acidic 
      phytohormones, including ten gibberellins (GAs), were well separated with a salt 
      gradient on poly(methacrylic acid-co-ethylene glycol dimethacrylate) 
      (MAA-co-EDMA) monolithic column. By employing online trapping system, the signal 
      intensities of the analytes were significantly improved. The limits of detection 
      (LODs, Signal/Noise=3) of targeted phytohormones ranged from 1.05 to 122.4 pg/mL, 
      which allowed the highly sensitive determination of low abundant acidic 
      phytohormones with tiny amount plant sample. Good reproducibility was obtained by 
      evaluating the intra- and inter-day precisions with relative standard deviations 
      (RSDs) less than 10.9 and 11.9%, respectively. Recoveries of the target analytes 
      from spiked rice leave samples ranged from 88.3 to 104.3%. By employing the 
      method developed here, we were able to simultaneously determine 11 endogenous 
      acidic phytohormones from only 5mg of rice leave sample, which dramatically 
      decreased the required sample amount (three orders of magnitude lower) for the 
      profiling of low abundant acidic phytohormones compared to previous reports. 
      Taken together, the method provided a good solution for the highly sensitive and 
      quantitative profiling of endogenous acidic phytohormones.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Chen, Ming-Luan
AU  - Chen ML
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Fu, Xiao-Meng
AU  - Fu XM
FAU - Liu, Jia-Qi
AU  - Liu JQ
FAU - Ye, Tian-Tian
AU  - Ye TT
FAU - Hou, Sheng-Yu
AU  - Hou SY
FAU - Huang, Yun-Qing
AU  - Huang YQ
FAU - Yuan, Bi-Feng
AU  - Yuan BF
FAU - Wu, Yan
AU  - Wu Y
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120813
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Ethylene Glycols)
RN  - 0 (Methacrylates)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (poly(methacrylic acid-ethylene glycol dimethacrylate))
SB  - IM
MH  - Chromatography, Liquid/instrumentation/*methods
MH  - Ethylene Glycols/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Methacrylates/chemistry
MH  - Nanotechnology
MH  - Oryza/chemistry
MH  - Plant Extracts/analysis/chemistry
MH  - Plant Growth Regulators/*analysis/chemistry
MH  - Plant Leaves/chemistry
MH  - Regression Analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tandem Mass Spectrometry/methods
EDAT- 2012/08/25 06:00
MHDA- 2013/03/01 06:00
CRDT- 2012/08/25 06:00
PHST- 2012/05/25 00:00 [received]
PHST- 2012/07/31 00:00 [revised]
PHST- 2012/08/03 00:00 [accepted]
PHST- 2012/08/25 06:00 [entrez]
PHST- 2012/08/25 06:00 [pubmed]
PHST- 2013/03/01 06:00 [medline]
AID - S1570-0232(12)00466-7 [pii]
AID - 10.1016/j.jchromb.2012.08.005 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Sep 15;905:67-74. doi: 
      10.1016/j.jchromb.2012.08.005. Epub 2012 Aug 13.