PMID- 22917617 OWN - NLM STAT- MEDLINE DCOM- 20130617 LR - 20211021 IS - 1873-7544 (Electronic) IS - 0306-4522 (Print) IS - 0306-4522 (Linking) VI - 224 DP - 2012 Nov 8 TI - Essential role of brain-derived neurotrophic factor in the regulation of serotonin transmission in the basolateral amygdala. PG - 125-34 LID - S0306-4522(12)00852-4 [pii] LID - 10.1016/j.neuroscience.2012.08.025 [doi] AB - Human and animal model studies have linked brain-derived neurotrophic factor (BDNF) with the etiology of anxiety disorders. This pleiotropic neurotrophin and its receptor, TrkB, promote neuronal survival, differentiation and synaptic plasticity. Here we interrogated the role of BDNF in serotonergic neurotransmission in the basolateral amygdala (BLA), a limbic brain region associated with the neurobiology of anxiety. We found that both GABAergic and pyramidal projection neurons in the wild-type BLA contained TrkB receptors. Examination of BDNF(2L/2LCk-Cre) mutant mice with brain-selective depletion of BDNF revealed mild decreases in serotonin content in the BLA. Notably, whole cell recordings in BLA pyramidal cells uncovered significant alterations in 5-HT(2)-mediated regulation of GABAergic and glutamatergic transmission in BDNF(2L/2LCk-Cre) mutant mice that result in a hyperexcitable circuit. These changes were associated with decreased expression of 5-HT(2) receptors. Collectively, the results indicate a required role of BDNF in serotonin transmission in the BLA. Furthermore, they suggest a mechanism underlying the reported increase in anxiety-like behavior elicited by perturbed BDNF signaling. CI - Copyright (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved. FAU - Daftary, S S AU - Daftary SS AD - Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA. FAU - Calderon, G AU - Calderon G FAU - Rios, M AU - Rios M LA - eng GR - P30 NS047243/NS/NINDS NIH HHS/United States GR - R01 DK073311/DK/NIDDK NIH HHS/United States GR - DK073311/DK/NIDDK NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20120821 PL - United States TA - Neuroscience JT - Neuroscience JID - 7605074 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 333DO1RDJY (Serotonin) SB - IM MH - Amygdala/*metabolism MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Chromatography, High Pressure Liquid MH - Immunohistochemistry MH - In Situ Hybridization MH - Male MH - Mice MH - Mice, Mutant Strains MH - Patch-Clamp Techniques MH - Pyramidal Cells/*metabolism MH - Reverse Transcriptase Polymerase Chain Reaction MH - Serotonin/*metabolism MH - Synaptic Transmission/*physiology PMC - PMC3475413 MID - NIHMS408948 EDAT- 2012/08/25 06:00 MHDA- 2013/06/19 06:00 PMCR- 2013/11/08 CRDT- 2012/08/25 06:00 PHST- 2012/05/16 00:00 [received] PHST- 2012/08/08 00:00 [revised] PHST- 2012/08/14 00:00 [accepted] PHST- 2012/08/25 06:00 [entrez] PHST- 2012/08/25 06:00 [pubmed] PHST- 2013/06/19 06:00 [medline] PHST- 2013/11/08 00:00 [pmc-release] AID - S0306-4522(12)00852-4 [pii] AID - 10.1016/j.neuroscience.2012.08.025 [doi] PST - ppublish SO - Neuroscience. 2012 Nov 8;224:125-34. doi: 10.1016/j.neuroscience.2012.08.025. Epub 2012 Aug 21.