PMID- 22919445
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20211021
IS  - 1948-9358 (Electronic)
IS  - 1948-9358 (Linking)
VI  - 3
IP  - 8
DP  - 2012 Aug 15
TI  - HLA-DQB1* alleles and genetic susceptibility to type 1 diabetes mellitus.
PG  - 149-55
LID - 10.4239/wjd.v3.i8.149 [doi]
AB  - AIM: To determine human leukocyte antigen (HLA)-DQB1 allele association with 
      susceptibility to type 1 diabetes (T1D) and to clinical and laboratory findings. 
      METHODS: This study was conducted on 85 unrelated Egyptian children with T1D 
      recruited consecutively from the Pediatric Diabetes Endocrinology outpatients 
      Clinic; Mansoura University Children's Hospital, Egypt. Patient mean follow up 
      period was 2.5 years. Patients were subdivided according to level of HbA1c 
      (optimal/suboptimal control < 8.5% and poor control >/= 8.5%). The control group 
      consisted of 113 unrelated age- and sex-matched healthy subjects without T1D or 
      other autoimmune diseases. Genomic DNA extraction was done for all subjects using 
      a DNA isolation kit. HLA-Class II-DQB1 allele typing was carried out with a 
      polymerase chain reaction-sequence-specific oligonucleotide probe using a 
      INNO-LiPA HLA-DQB1 update kit. RESULTS: Significant differences were detected 
      between Egyptian patients with T1D and control groups in the frequencies of 
      DQB1*02 [44.4% vs 18.6%, corrected P value (Pc) < 0.001] and DQB1*03 (41.2% vs 
      24.4%, Pc < 0.001). Significant differences were also observed between control 
      groups and T1D patients in the frequencies of DQB1*05 (14.6% vs 7.2%, P = 0.029) 
      and DQB1*06 (34.1% vs 7.2%, P < 0.001). However, after correction for multiple 
      comparisons, the significance was retained for HLA-DQB1*06 (Pc < 0.001) but lost 
      for HLA-DQB1*05. HLA-DQB1*0201, *0202, *030201 were positively associated with 
      T1D (Pc = 0.014, Pc < 0.001, and Pc < 0.001 respectively), while HLA-DQB1*060101 
      was negatively associated (Pc < 0.001) with the condition. Although the HLA-DQB1 
      alleles 030101 and 050101 were significantly higher in controls (P = 0.016, P = 
      0.025 respectively), both of them lost statistical significance after correction 
      of P value. The frequency of the HLA-DQB1 genotypes 02/02, 02/03, and 03/03 was 
      higher in T1D patients, and the frequency of the genotypes 03/06, 05/06, and 
      06/06 was higher in controls, these differences being statistically significant 
      before correction. After correction, the genotypes 02/02, 02/03 in T1D, and the 
      genotypes 03/06, 06/06 in controls were still significant (Pc = 0.01, Pc < 0.001, 
      Pc < 0.001, and Pc = 0.04, respectively). Non-significant associations were found 
      between the frequency HLA-DQB1 alleles and genotypes in T1D in relation to the 
      grade of diabetic control, Microalbuminuria, age, gender, age of presentation, 
      weight, height, frequency of diabetic ketoacidosis (P = 0.42), serum cholesterol, 
      and fasting and post-prandial level of C-peptide (P = 0.83, P = 0.9, 
      respectively). CONCLUSION: The Current work suggests that HLA-DQB1 alleles 
      *030201, *0202, *0201, and genotypes 02/03, 02/02 may be susceptibility risk 
      factors for development of T1D in Egyptian children, while the HLA-DQB1*060101 
      allele, and 03/06, 06/06 genotypes may be protective factors. HLA-DQB1 alleles 
      and genotypes do not contribute to microalbuminuria or grade of diabetic control.
FAU - Mosaad, Youssef M
AU  - Mosaad YM
AD  - Youssef M Mosaad, Fatma A Auf, Shereen S Metwally, Ziyad E Tawhid, Farha A 
      El-Chennawi, Unit of Clinical Immunology, Department of Clinical Pathology, 
      Mansoura Faculty of Medicine, Mansoura 35111, Egypt.
FAU - Auf, Fatma A
AU  - Auf FA
FAU - Metwally, Shereen S
AU  - Metwally SS
FAU - Elsharkawy, Ashraf A
AU  - Elsharkawy AA
FAU - El-Hawary, Amany K
AU  - El-Hawary AK
FAU - Hassan, Rasha H
AU  - Hassan RH
FAU - Tawhid, Ziyad E
AU  - Tawhid ZE
FAU - El-Chennawi, Farha A
AU  - El-Chennawi FA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Diabetes
JT  - World journal of diabetes
JID - 101547524
PMC - PMC3425629
OTO - NOTNLM
OT  - Children, Complication
OT  - Egyptian
OT  - Genetic susceptibility
OT  - HLA-DQB1
OT  - Type 1 diabetes
EDAT- 2012/08/25 06:00
MHDA- 2012/08/25 06:01
PMCR- 2012/08/15
CRDT- 2012/08/25 06:00
PHST- 2012/03/19 00:00 [received]
PHST- 2012/06/27 00:00 [revised]
PHST- 2012/08/08 00:00 [accepted]
PHST- 2012/08/25 06:00 [entrez]
PHST- 2012/08/25 06:00 [pubmed]
PHST- 2012/08/25 06:01 [medline]
PHST- 2012/08/15 00:00 [pmc-release]
AID - 10.4239/wjd.v3.i8.149 [doi]
PST - ppublish
SO  - World J Diabetes. 2012 Aug 15;3(8):149-55. doi: 10.4239/wjd.v3.i8.149.