PMID- 22921811
OWN - NLM
STAT- MEDLINE
DCOM- 20130110
LR  - 20220317
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1820
IP  - 12
DP  - 2012 Dec
TI  - Cytosolic ascorbate peroxidase 1 protects organelles against oxidative stress by 
      wounding- and jasmonate-induced H(2)O(2) in Arabidopsis plants.
PG  - 1901-7
LID - S0304-4165(12)00223-1 [pii]
LID - 10.1016/j.bbagen.2012.08.003 [doi]
AB  - BACKGROUND: Reactive oxygen species (ROS) are not only cytotoxic compounds 
      leading to oxidative damage, but also signaling molecules for regulating plant 
      responses to stress and hormones. Arabidopsis cytosolic ascorbate peroxidase 1 
      (APX1) is thought to be a central regulator for cellular ROS levels. However, it 
      remains unclear whether APX1 is involved in plant tolerance to wounding and 
      methyl jasmonate (MeJA) treatment, which are known to enhance ROS production. 
      METHODS: We studied the effect of wounding and MeJA treatment on the levels of 
      H(2)O(2) and oxidative damage in the Arabidopsis wild-type plants and knockout 
      mutants lacking APX1 (KO-APX1). RESULTS: The KO-APX1 plants showed high 
      sensitivity to wounding and MeJA treatment. In the leaves of wild-type plants, 
      H(2)O(2) accumulated only in the vicinity of the wound, while in the leaves of 
      the KO-APX1 plants it accumulated extensively from damaged to undamaged regions. 
      During MeJA treatment, the levels of H(2)O(2) were much higher in the leaves of 
      KO-APX1 plants. Oxidative damage in the chloroplasts and nucleus was also 
      enhanced in the leaves of KO-APX1 plants. These findings suggest that APX1 
      protects organelles against oxidative stress by wounding and MeJA treatment. 
      GENERAL SIGNIFICANCE: This is the first report demonstrating that 
      H(2)O(2)-scavenging in the cytosol is essential for plant tolerance to wounding 
      and MeJA treatment.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Maruta, Takanori
AU  - Maruta T
AD  - Department of Advanced Bioscience, Kinki University, Nara, Japan.
FAU - Inoue, Takahiro
AU  - Inoue T
FAU - Noshi, Masahiro
AU  - Noshi M
FAU - Tamoi, Masahiro
AU  - Tamoi M
FAU - Yabuta, Yukinori
AU  - Yabuta Y
FAU - Yoshimura, Kazuya
AU  - Yoshimura K
FAU - Ishikawa, Takahiro
AU  - Ishikawa T
FAU - Shigeoka, Shigeru
AU  - Shigeoka S
LA  - eng
PT  - Journal Article
DEP - 20120818
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Acetates)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cyclopentanes)
RN  - 0 (Oxylipins)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Plant)
RN  - 0 (Reactive Oxygen Species)
RN  - 1406-65-1 (Chlorophyll)
RN  - 900N171A0F (methyl jasmonate)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.11 (APX1 protein, Arabidopsis)
RN  - EC 1.11.1.11 (Ascorbate Peroxidases)
SB  - IM
MH  - Acetates/*pharmacology
MH  - Arabidopsis/*enzymology/genetics
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Ascorbate Peroxidases/genetics/*metabolism
MH  - Blotting, Western
MH  - Chlorophyll/metabolism
MH  - Cyclopentanes/*pharmacology
MH  - Cytosol/*enzymology
MH  - Hydrogen Peroxide/*metabolism
MH  - Organelles/drug effects/*physiology
MH  - Oxidative Stress/drug effects/*physiology
MH  - Oxylipins/*pharmacology
MH  - Plant Diseases/genetics/prevention & control
MH  - Plant Growth Regulators/pharmacology
MH  - Plants, Genetically Modified/enzymology/genetics
MH  - RNA, Messenger/genetics
MH  - RNA, Plant/genetics
MH  - Reactive Oxygen Species/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Wound Healing
EDAT- 2012/08/28 06:00
MHDA- 2013/01/11 06:00
CRDT- 2012/08/28 06:00
PHST- 2012/05/24 00:00 [received]
PHST- 2012/07/31 00:00 [revised]
PHST- 2012/08/07 00:00 [accepted]
PHST- 2012/08/28 06:00 [entrez]
PHST- 2012/08/28 06:00 [pubmed]
PHST- 2013/01/11 06:00 [medline]
AID - S0304-4165(12)00223-1 [pii]
AID - 10.1016/j.bbagen.2012.08.003 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2012 Dec;1820(12):1901-7. doi: 
      10.1016/j.bbagen.2012.08.003. Epub 2012 Aug 18.