PMID- 22922140
OWN - NLM
STAT- MEDLINE
DCOM- 20130502
LR  - 20220317
IS  - 1732-2693 (Electronic)
IS  - 0032-5449 (Linking)
VI  - 66
DP  - 2012 Jun 20
TI  - The impact of IL18 gene polymorphisms on mRNA levels and interleukin-18 release 
      by peripheral blood mononuclear cells.
PG  - 409-14
AB  - INTRODUCTION: Interleukin-18 (IL-18) is a pleiotropic cytokine playing an 
      important role as a modulator of immune responses, found to play a role in 
      pathogenesis of numerous inflammatory-associated disorders. In the present study 
      a potential association between 7 common single-nucleotide polymorphisms (SNPs) 
      spanning the whole IL18 gene, gene expression and the release of IL-18 from the 
      stimulated peripheral blood mononuclear cells (PBMCs) was investigated. 
      MATERIALS/METHODS: PBMCs were isolated from peripheral blood of 29 healthy 
      volunteers, genotyped for the presence of IL18 SNPs: rs1946518: A>C, rs187238: 
      G>C, rs360718: A>C, rs360722: C>T, rs360721: C>G, rs549908: T>G, and rs5744292: 
      A>G. IL-18 concentration and IL18 mRNA levels were investigated after incubation 
      of cells for 48 h with different stimulants (PHA, LPS, and anti-CD3/CD28 
      antibodies). RESULTS: After treatment with LPS and antibodies IL-18 
      concentrations were significantly lower in rs1946518AA homozygotes than in C 
      allele carriers. When differences in IL18 mRNA levels between non-stimulated and 
      stimulated cells were analyzed, significantly decreased gene expression was noted 
      in rs1946518 AA homozygotes (as compared with C allele carriers) in samples 
      treated with PHA and LPS. Similar trends were observed in the case of rs187238 
      SNP; however, the differences reached statistical significance only after PHA 
      treatment. CONCLUSIONS: Our study supports the role of rs1946518 (-607A>C) and 
      rs187238 (-137G>C) SNPs as genetic determinants of the observed variability in 
      IL18 expression.
FAU - Dziedziejko, Violetta
AU  - Dziedziejko V
AD  - Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, 
      Szczecin, Poland.
FAU - Kurzawski, Mateusz
AU  - Kurzawski M
FAU - Paczkowska, Edyta
AU  - Paczkowska E
FAU - Machalinski, Boguslaw
AU  - Machalinski B
FAU - Pawlik, Andrzej
AU  - Pawlik A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120620
PL  - Poland
TA  - Postepy Hig Med Dosw (Online)
JT  - Postepy higieny i medycyny doswiadczalnej (Online)
JID - 101206517
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Alleles
MH  - Female
MH  - Gene Expression
MH  - Genotype
MH  - Heterozygote
MH  - Homozygote
MH  - Humans
MH  - Interleukin-18/*genetics/*metabolism
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - *Polymorphism, Single Nucleotide
MH  - RNA, Messenger/*metabolism
EDAT- 2012/08/28 06:00
MHDA- 2013/05/03 06:00
CRDT- 2012/08/28 06:00
PHST- 2012/08/28 06:00 [entrez]
PHST- 2012/08/28 06:00 [pubmed]
PHST- 2013/05/03 06:00 [medline]
AID - 1000980 [pii]
AID - 10.5604/17322693.1000980 [doi]
PST - epublish
SO  - Postepy Hig Med Dosw (Online). 2012 Jun 20;66:409-14. doi: 
      10.5604/17322693.1000980.