PMID- 22922352
OWN - NLM
STAT- MEDLINE
DCOM- 20130617
LR  - 20121010
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 224
DP  - 2012 Nov 8
TI  - Brain-derived neurotrophic factor increases the motility of a particular 
      N-methyl-D-aspartate /GABA-responsive subset of neural progenitor cells.
PG  - 223-34
LID - S0306-4522(12)00865-2 [pii]
LID - 10.1016/j.neuroscience.2012.08.038 [doi]
AB  - Neurotrophins like brain-derived neurotrophic factor (BDNF) promote the migration 
      of subsets of neural progenitor cells. The mechanism by which motility is 
      increased and the functional properties of BDNF-responsive cells are not very 
      well known. We have used the neurosphere model, combining time-lapse microscopy, 
      immunocytochemistry, and Ca(2+) imaging, to study the effect of BDNF on 
      parameters such as motility and neurotransmitter responsiveness of migrating 
      neural progenitors. At the initiation of differentiation thick glial 
      glutamate-aspartate transporter (GLAST)-positive radial processes emerged from 
      the neurosphere, followed by the exit of neuron-like cells. The neuron-like cells 
      moved outside the radial processes in a phasic manner with intermittent surges of 
      motility and stationary periods. BDNF increased the number and promoted the 
      progress of the neuron-like cells by prolonging surges and decreasing the length 
      of stationary phases. The average rate of cellular movement during surges was 
      unaffected by BDNF. BDNF also caused a several fold increase in positive staining 
      for tropomyosin-related kinase B (TrkB) receptors and neuronal markers such as 
      Calbindin, microtubule-associated protein-2 (MAP-2), and neuron-specific nuclear 
      protein (NeuN) in cells outside the radial network. Calcium imaging allowed for 
      further characterization of the BDNF-responsive cell population. 
      Kainate-responsive cells, denoting the expression of AMPA/kainate receptors, 
      dominated in the outer migration layers while cells responding to 
      (S)-3,5-dihydroxyphenylglycine (DHPG) via metabotropic glutamate receptor 5 
      (mGluR5) dominated in the inner migration layers. BDNF did not appreciably affect 
      the distribution of these cells but promoted the redistribution of a small 
      subpopulation (about 20%) of N-methyl-D-aspartate (NMDA)- and GABA-responsive 
      cells to the outermost layers of migration. The results demonstrate that BDNF 
      does not affect cell motility per se but alters the phasic behavior of cell 
      movement by promoting periods of high motility in a defined subpopulation of 
      cells which give a robust Ca(2+) response to NMDA and GABA.
CI  - Copyright (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Jansson, L C
AU  - Jansson LC
AD  - Biomedicum Helsinki, Institute of Biomedicine/Physiology, PO Box 63, University 
      of Helsinki, FIN-00014 Helsinki, Finland.
FAU - Louhivuori, L
AU  - Louhivuori L
FAU - Wigren, H-K
AU  - Wigren HK
FAU - Nordstrom, T
AU  - Nordstrom T
FAU - Louhivuori, V
AU  - Louhivuori V
FAU - Castren, M L
AU  - Castren ML
FAU - Akerman, K E
AU  - Akerman KE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120823
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Movement/*physiology
MH  - Cells, Cultured
MH  - Immunohistochemistry
MH  - Mice
MH  - N-Methylaspartate/metabolism
MH  - Neural Stem Cells/*cytology/metabolism
MH  - gamma-Aminobutyric Acid/metabolism
EDAT- 2012/08/28 06:00
MHDA- 2013/06/19 06:00
CRDT- 2012/08/28 06:00
PHST- 2012/05/30 00:00 [received]
PHST- 2012/08/15 00:00 [revised]
PHST- 2012/08/16 00:00 [accepted]
PHST- 2012/08/28 06:00 [entrez]
PHST- 2012/08/28 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
AID - S0306-4522(12)00865-2 [pii]
AID - 10.1016/j.neuroscience.2012.08.038 [doi]
PST - ppublish
SO  - Neuroscience. 2012 Nov 8;224:223-34. doi: 10.1016/j.neuroscience.2012.08.038. 
      Epub 2012 Aug 23.