PMID- 22922428
OWN - NLM
STAT- MEDLINE
DCOM- 20130510
LR  - 20181202
IS  - 1873-3441 (Electronic)
IS  - 0939-6411 (Linking)
VI  - 82
IP  - 3
DP  - 2012 Nov
TI  - PLGA microspheres encapsulating siRNA anti-TNFalpha: efficient RNAi-mediated 
      treatment of arthritic joints.
PG  - 457-64
LID - S0939-6411(12)00248-2 [pii]
LID - 10.1016/j.ejpb.2012.07.021 [doi]
AB  - The aim of this study was to investigate potentialities of 
      poly(dl-lactide-co-glycolide) (PLGA) microspheres for the delivery of small 
      interfering RNAs (siRNAs) against tumor necrosis factor alpha (TNF-alpha) to achieve 
      prolonged and efficient inhibition of TNF-alpha for the treatment of rheumatoid 
      arthritis (RA). PLGA microspheres were prepared by a modified multiple 
      emulsion-solvent evaporation method. The formulations were characterized in terms 
      of morphology, mean diameter and siRNAs distribution, encapsulation efficiency, 
      and in vitro release kinetics. The efficiency of this system was then evaluated 
      both in vitro and in vivo using the murine monocytic cell line J774 and a 
      pre-clinical model of RA, respectively. siRNA-encapsulating PLGA microspheres 
      were characterized by a high encapsulation efficiency and a slow and prolonged 
      anti-TNF-alpha siRNAs. Our results provide evidence that, upon intra-articular 
      administration, PLGA microspheres slowly releasing siRNAs effectively inhibited 
      the expression of TNF-alpha in arthritic joints. Our system might represent an 
      alternative strategy for the design of novel anti-rheumatic therapies based on 
      the use of RNA interference in RA.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Presumey, J
AU  - Presumey J
AD  - Inserm, U 844, INM, Hopital Saint Eloi, Montpellier, France.
FAU - Salzano, G
AU  - Salzano G
FAU - Courties, G
AU  - Courties G
FAU - Shires, M
AU  - Shires M
FAU - Ponchel, F
AU  - Ponchel F
FAU - Jorgensen, C
AU  - Jorgensen C
FAU - Apparailly, F
AU  - Apparailly F
FAU - De Rosa, G
AU  - De Rosa G
LA  - eng
PT  - Journal Article
DEP - 20120816
PL  - Netherlands
TA  - Eur J Pharm Biopharm
JT  - European journal of pharmaceutics and biopharmaceutics : official journal of 
      Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
JID - 9109778
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*pathology/therapy
MH  - Arthritis, Rheumatoid/*pathology/therapy
MH  - Cell Line
MH  - Drug Design
MH  - Lactic Acid/chemistry
MH  - Mice
MH  - Mice, Inbred DBA
MH  - Microspheres
MH  - Particle Size
MH  - Polyglycolic Acid/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - RNA Interference
MH  - RNA, Small Interfering/*administration & dosage
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2012/08/28 06:00
MHDA- 2013/05/11 06:00
CRDT- 2012/08/28 06:00
PHST- 2012/04/10 00:00 [received]
PHST- 2012/07/30 00:00 [revised]
PHST- 2012/07/31 00:00 [accepted]
PHST- 2012/08/28 06:00 [entrez]
PHST- 2012/08/28 06:00 [pubmed]
PHST- 2013/05/11 06:00 [medline]
AID - S0939-6411(12)00248-2 [pii]
AID - 10.1016/j.ejpb.2012.07.021 [doi]
PST - ppublish
SO  - Eur J Pharm Biopharm. 2012 Nov;82(3):457-64. doi: 10.1016/j.ejpb.2012.07.021. 
      Epub 2012 Aug 16.