PMID- 22922992
OWN - NLM
STAT- MEDLINE
DCOM- 20130307
LR  - 20211203
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 30
IP  - 5
DP  - 2012 Nov
TI  - Sage weed (Salvia plebeia) extract antagonizes foam cell formation and promotes 
      cholesterol efflux in murine macrophages.
PG  - 1105-12
LID - 10.3892/ijmm.2012.1103 [doi]
AB  - Lipid-laden peripheral tissue cells release cholesterol to an extracellular 
      acceptor such as high-density lipoprotein (HDL). Foam cells are formed at the 
      first stage of atherosclerosis development. This study investigated whether sage 
      weed (Salvia plebeia) extract (SWE) influences cholesterol handling of J774A1 
      murine macrophages. A murine macrophage cell line, J774A1, was used in this 
      study. Oxidized low-density lipoproteins (LDL) treatment was used for foam cell 
      formation, which was confirmed using Oil red O staining. The oxidized LDL uptake 
      and cholesterol efflux from lipid-laden foam cell-associated proteins were 
      detected by western blot analysis. Also, transcriptional levels of these 
      associated genes were examined using reverse transcription-PCR. Also, cholesterol 
      efflux was measured using NBD-cholesterol efflux assay. Non-toxic SWE at >/=10 
      microg/ml attenuated scavenger receptor (SR)-B1 expression of macrophages induced by 
      oxidized LDL for 6 h, which was achieved at its transcriptional levels. 
      Consistently, SWE suppressed oxidized LDL-stimulated cellular lipid accumulation 
      and foam cell formation due to downregulated SR-B1. SWE upregulated the protein 
      expression and mRNA levels of ATP-binding cassette transporter A1 (ABCA1) and 
      ATP-binding cassette transporter G1 (ABCG1) in lipid-laden foam cells, both 
      responsible for cholesterol efflux. In addition, SWE promoted apolipoprotein E 
      (apoE) secretion from oxidized LDL-induced foam cells. Cholesterol efflux was 
      enhanced by >/=10 microg/ml SWE most likely through the induction of ABCA1 and ABCG1 
      and the secretion of apoE. Although 10 microM homoplantaginin, a compound mainly 
      present in sage weeds, did not influence cellular expression of ABCA1 and ABCG1, 
      it suppressed oxidized LDL-enhanced SR-B1 induction and foam cell formation. 
      These results demonstrate that SWE antagonized oxidized LDL uptake and promoted 
      cholesterol efflux in lipid-laden macrophages. Therefore, SWE may serve as a 
      protective therapeutic agent against the development of atherosclerosis.
FAU - Park, Sin-Hye
AU  - Park SH
AD  - Department of Food and Nutrition, Hallym University, Chuncheon 200-702, Republic 
      of Korea.
FAU - Kim, Jung-Lye
AU  - Kim JL
FAU - Kang, Min-Kyung
AU  - Kang MK
FAU - Gong, Ju-Hyun
AU  - Gong JH
FAU - Han, Seon-Young
AU  - Han SY
FAU - Shim, Jae-Hoon
AU  - Shim JH
FAU - Lim, Soon Sung
AU  - Lim SS
FAU - Kang, Young-Hee
AU  - Kang YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120820
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (6-methoxy-4',5-dihydroxyflavone-7-O-glucoside)
RN  - 0 (ABCA1 protein, human)
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Apolipoproteins E)
RN  - 0 (Camphanes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavones)
RN  - 0 (Flavonoids)
RN  - 0 (Glucosides)
RN  - 0 (Lipoproteins)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Scarb1 protein, mouse)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 0 (danshen dripping pill)
RN  - 0 (oxidized low density lipoprotein)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - N7F61604C2 (hispidulin)
SB  - IM
MH  - ATP Binding Cassette Transporter 1
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/genetics/metabolism
MH  - Animals
MH  - Apolipoproteins E/metabolism
MH  - Camphanes
MH  - Cell Line
MH  - Cholesterol/*metabolism
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Flavones/pharmacology
MH  - Flavonoids/pharmacology
MH  - Foam Cells/*drug effects
MH  - Gene Expression/drug effects
MH  - Glucosides/pharmacology
MH  - Humans
MH  - Lipoproteins/genetics/metabolism
MH  - Lipoproteins, LDL/metabolism/physiology
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Panax notoginseng
MH  - Salvia/*chemistry
MH  - Salvia miltiorrhiza
MH  - Scavenger Receptors, Class B/genetics/metabolism
MH  - Transcriptional Activation/drug effects
EDAT- 2012/08/28 06:00
MHDA- 2013/03/08 06:00
CRDT- 2012/08/28 06:00
PHST- 2012/05/21 00:00 [received]
PHST- 2012/07/27 00:00 [accepted]
PHST- 2012/08/28 06:00 [entrez]
PHST- 2012/08/28 06:00 [pubmed]
PHST- 2013/03/08 06:00 [medline]
AID - 10.3892/ijmm.2012.1103 [doi]
PST - ppublish
SO  - Int J Mol Med. 2012 Nov;30(5):1105-12. doi: 10.3892/ijmm.2012.1103. Epub 2012 Aug 
      20.