PMID- 22924020
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1380-3743 (Print)
IS  - 1572-9788 (Electronic)
IS  - 1380-3743 (Linking)
VI  - 30
IP  - 2
DP  - 2012 Aug
TI  - Genetic characterization of a reciprocal translocation present in a widely grown 
      barley variety.
PG  - 1109-1119
AB  - Artificially induced translocation stocks have been used to physically map the 
      barley genome; however, natural translocations are extremely uncommon in 
      cultivated genotypes. Albacete is a barley variety widely grown in recent decades 
      in Spain and carrying a reciprocal translocation which obviously does not affect 
      its agronomical fitness. This translocation has been characterized by a 
      combination of cytological and molecular genetic approaches. Firstly, 
      recombination frequencies between markers on chromosomes 1H and 3H were estimated 
      to determine the boundaries of the reciprocal interchange. Secondly, 1H-3H wheat 
      barley telosome addition lines were used to assign selected markers to chromosome 
      arms. Thirdly, fluorescence in situ hybridization (FISH) with rDNA probes (5S and 
      18S-5.8S-26S) and microsatellite probes [(ACT)(5), (AAG)(5) and (CAG)(5)] was 
      used to determine the locations of the translocation breakpoints more precisely. 
      Fourthly, fine-mapping of the regions around the translocation breakpoints was 
      used to increase the marker density for comparative genomics. The results 
      obtained in this study indicate that the translocation is quite large with 
      breakpoints located on the long arms of chromosomes 1H and 3H, between the 
      pericentromeric (AAG)(5) bands and above the (ACT)(5) interstitial distal bands, 
      resulting in the reciprocal translocation 1HS.1HL-3HL and 3HS.3HL-1HL. The gene 
      content around the translocation breakpoints could be inferred from syntenic 
      relationships observed among different species from the grass family Poaceae 
      (rice, Sorghum and Brachypodium) and was estimated at approximately 1,100 and 710 
      gene models for 1H and 3H, respectively. Duplicated segments between chromosomes 
      Os01 and Os05 in rice derived from ancestral duplications within the grass family 
      overlap with the translocation breakpoints on chromosomes 1H and 3H in the barley 
      variety Albacete.
FAU - Farre, A
AU  - Farre A
FAU - Cuadrado, A
AU  - Cuadrado A
FAU - Lacasa-Benito, I
AU  - Lacasa-Benito I
FAU - Cistue, L
AU  - Cistue L
FAU - Schubert, I
AU  - Schubert I
FAU - Comadran, J
AU  - Comadran J
FAU - Jansen, J
AU  - Jansen J
FAU - Romagosa, I
AU  - Romagosa I
LA  - eng
PT  - Journal Article
DEP - 20120128
PL  - Netherlands
TA  - Mol Breed
JT  - Molecular breeding : new strategies in plant improvement
JID - 9506703
PMC - PMC3410021
EDAT- 2012/08/28 06:00
MHDA- 2012/08/28 06:01
PMCR- 2012/01/28
CRDT- 2012/08/28 06:00
PHST- 2011/09/29 00:00 [received]
PHST- 2011/12/27 00:00 [accepted]
PHST- 2012/08/28 06:00 [entrez]
PHST- 2012/08/28 06:00 [pubmed]
PHST- 2012/08/28 06:01 [medline]
PHST- 2012/01/28 00:00 [pmc-release]
AID - 9698 [pii]
AID - 10.1007/s11032-011-9698-z [doi]
PST - ppublish
SO  - Mol Breed. 2012 Aug;30(2):1109-1119. doi: 10.1007/s11032-011-9698-z. Epub 2012 
      Jan 28.