PMID- 22925160 OWN - NLM STAT- MEDLINE DCOM- 20140211 LR - 20211021 IS - 1582-4934 (Electronic) IS - 1582-1838 (Print) IS - 1582-1838 (Linking) VI - 16 IP - 12 DP - 2012 Dec TI - BDNF-mediated migration of cardiac microvascular endothelial cells is impaired during ageing. PG - 3105-15 LID - 10.1111/j.1582-4934.2012.01621.x [doi] AB - This study indicates that brain-derived neurotrophic factor (BDNF) can promote young cardiac microvascular endothelial cells (CMECs) to migrate via the activation of the BDNF-TrkB-FL-PI3K/Akt pathway, which may benefit angiogenesis after myocardial infarction (MI). However, the ageing of CMECs led to changes in the expression of receptor Trk isoforms in that among the three isoforms (TrkB-FL, TrkB-T1 and TrkB-T2), only one of its truncated isoforms, TrkB-T1, continued to be expressed, which leads to the dysfunction of its ligand, a decrease in the migration of CMECs and increased injury in ageing hearts. This shift in receptor isoforms in aged CMECs, together with changes in the ageing microenvironment, might predispose ageing hearts to decreased angiogenic potential and increased cardiac pathology. CI - (c) 2012 The Authors Journal of Cellular and Molecular Medicine (c) 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd. FAU - Cao, Liang AU - Cao L AD - Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China. FAU - Zhang, Liang AU - Zhang L FAU - Chen, Siyun AU - Chen S FAU - Yuan, Ziqiang AU - Yuan Z FAU - Liu, Shaokun AU - Liu S FAU - Shen, Xiaotao AU - Shen X FAU - Zheng, Xin AU - Zheng X FAU - Qi, Xufeng AU - Qi X FAU - Lee, Kenneth K H AU - Lee KK FAU - Chan, John Yeuk-Hon AU - Chan JY FAU - Cai, Dongqing AU - Cai D LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Cell Mol Med JT - Journal of cellular and molecular medicine JID - 101083777 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Protein Isoforms) RN - EC 2.7.1.- (Phosphatidylinositol 3-Kinases) RN - EC 2.7.10.1 (Receptor, trkB) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) SB - IM MH - *Aging MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - *Cell Movement MH - Endothelial Cells/*physiology MH - Female MH - Heart MH - Myocardial Infarction MH - Neovascularization, Physiologic/*physiology MH - Phosphatidylinositol 3-Kinases/metabolism MH - Protein Isoforms/metabolism MH - Proto-Oncogene Proteins c-akt/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, trkB/*metabolism MH - Signal Transduction PMC - PMC4393738 EDAT- 2012/08/29 06:00 MHDA- 2014/02/12 06:00 PMCR- 2012/12/01 CRDT- 2012/08/29 06:00 PHST- 2012/06/25 00:00 [received] PHST- 2012/08/16 00:00 [accepted] PHST- 2012/08/29 06:00 [entrez] PHST- 2012/08/29 06:00 [pubmed] PHST- 2014/02/12 06:00 [medline] PHST- 2012/12/01 00:00 [pmc-release] AID - 10.1111/j.1582-4934.2012.01621.x [doi] PST - ppublish SO - J Cell Mol Med. 2012 Dec;16(12):3105-15. doi: 10.1111/j.1582-4934.2012.01621.x.