PMID- 22925896
OWN - NLM
STAT- MEDLINE
DCOM- 20130207
LR  - 20121002
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 426
IP  - 3
DP  - 2012 Sep 28
TI  - WT1 CpG islands methylation in human lung cancer: a pilot study.
PG  - 306-9
LID - S0006-291X(12)01569-0 [pii]
LID - 10.1016/j.bbrc.2012.08.059 [doi]
AB  - BACKGROUND: CpG island hypermethylation of gene promoters and regulatory regions 
      is a well-known mechanism of epigenetic silencing of tumor suppressors and is 
      directly linked to carcinogenesis. Wilm's tumor gene (WT1) is a tumor suppressor 
      protein involved in the regulation of human cell growth and differentiation and a 
      modulator of oncogenic K Ras signaling in lung cancer. Changes in the pattern of 
      methylation of the WT1 gene have not yet been studied in detail in human lung 
      cancer. In this study we compared the methylation profile of WT1 gene in samples 
      of neoplastic and non-neoplastic lung tissue taken from the same patients. 
      METHODS: DNA was extracted from neoplastic and normal lung tissue obtained from 
      16 patients with non small cell lung cancer (NSCLC). The methylation status of 29 
      CpG islands in the 5' region of WT1 was determined by pyrosequencing. Statistical 
      analysis was carried out by T test and Mann Whitney test. RESULTS: The mean 
      percentage of methylation, considering all CpG islands of WT1 in the neoplastic 
      tissues of the 16 NSCLC patients, was 16.2 +/- 3.4, whereas in the normal lung 
      tissue from the same patients it was 5.6 +/- 1.7 (p < 0.001). Adenocarcinomas 
      presented higher methylation levels than squamous cell carcinomas (p < 0,001). 
      CONCLUSIONS: Methylation of WT1 gene is significantly increased in NSCLC. Both 
      histotype and exposure to cigarette smoke heavily influence the pattern of CpG 
      islands which undergo hypermethylation.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Bruno, Pierdonato
AU  - Bruno P
AD  - Department of Clinic and Molecular Medicine, Sapienza University of Rome, Italy.
FAU - Gentile, Giovanna
AU  - Gentile G
FAU - Mancini, Rita
AU  - Mancini R
FAU - De Vitis, Claudia
AU  - De Vitis C
FAU - Esposito, Maria Cristina
AU  - Esposito MC
FAU - Scozzi, Davide
AU  - Scozzi D
FAU - Mastrangelo, Mario
AU  - Mastrangelo M
FAU - Ricci, Alberto
AU  - Ricci A
FAU - Mohsen, Ibrahim
AU  - Mohsen I
FAU - Ciliberto, Gennaro
AU  - Ciliberto G
FAU - Simmaco, Maurizio
AU  - Simmaco M
FAU - Mariotta, Salvatore
AU  - Mariotta S
LA  - eng
PT  - Journal Article
DEP - 20120820
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (WT1 Proteins)
SB  - IM
MH  - Adenocarcinoma/genetics/pathology
MH  - Aged
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Cell Transformation, Neoplastic/genetics
MH  - *CpG Islands
MH  - *DNA Methylation
MH  - *Gene Expression Regulation, Neoplastic
MH  - *Gene Silencing
MH  - Humans
MH  - Lung/metabolism/pathology
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Pilot Projects
MH  - Smoking/genetics
MH  - WT1 Proteins/*genetics
EDAT- 2012/08/29 06:00
MHDA- 2013/02/08 06:00
CRDT- 2012/08/29 06:00
PHST- 2012/08/03 00:00 [received]
PHST- 2012/08/13 00:00 [accepted]
PHST- 2012/08/29 06:00 [entrez]
PHST- 2012/08/29 06:00 [pubmed]
PHST- 2013/02/08 06:00 [medline]
AID - S0006-291X(12)01569-0 [pii]
AID - 10.1016/j.bbrc.2012.08.059 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Sep 28;426(3):306-9. doi: 
      10.1016/j.bbrc.2012.08.059. Epub 2012 Aug 20.