PMID- 22926256 OWN - NLM STAT- MEDLINE DCOM- 20130304 LR - 20121001 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 33 IP - 4 DP - 2012 Oct TI - Hematological parameters and phagocytic activity in fat snook (Centropomus parallelus) bred in captivity. PG - 953-61 LID - S1050-4648(12)00290-2 [pii] LID - 10.1016/j.fsi.2012.08.005 [doi] AB - The objective of this work was to determine the hematological parameters and the phagocytic capacity of peritoneal macrophages of fat snook related to sex, stage of gonadal maturation and seasonal cycle. Blood was collected from 135 animals (78 females and 57 males) and used for determinations of: erythrocyte number, hematocrit, hemoglobin, erythrocyte indices mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), total and differential leukocyte counts, and thrombocyte count. The phagocytic capacity and phagocytic index were determined after Saccharomyces cerevisiae inoculation in the peritoneal cavity of the animals. The hematological results according to sex showed that the erythrocyte, total leukocyte and thrombocyte counts were statistically higher in males than females, with the latter showing a higher MCV. Concerning to erythrocyte count, hematocrit and hemoglobin concentration analyzed separately by sex and stage of gonadal maturation, males were found to have significantly elevated values in the mature stage and decreased levels in the resting stage. The results of the erythrocyte and leukocyte series, thrombocytes and phagocytic activity related to seasonal cycle showed significant differences in both sexes, where hematocrit and hemoglobin concentration were lower in winter and higher in the other seasons, mean corpuscular volume was higher in the summer and lower in the winter and fall, total leukocytes and thrombocytes lower in the spring and higher in the fall, lymphocytes low in the winter and summer and high in the spring and phagocytic capacity and phagocytic index high in the summer and low in the winter and fall. The results showed that the hematological values in males are statistically higher than those in females, the erythrocyte values in males increase with the progression of gonadal maturation and that winter is the season of the year least favorable for hematological and phagocytic responses for survival of fat snook kept in captivity. The parameters studied could be utilized in the evaluation of the health status of this species in captivity. CI - Copyright (c) 2012 Elsevier Ltd. All rights reserved. FAU - Santos, Antenor Aguiar AU - Santos AA AD - UNIFESP/EPM, SP, Brazil. antenorbio@gmail.com FAU - Ranzani-Paiva, Maria Jose T AU - Ranzani-Paiva MJ FAU - da Veiga, Marcelo Leite AU - da Veiga ML FAU - Faustino, Lucas AU - Faustino L FAU - Egami, Mizue I AU - Egami MI LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120821 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 SB - IM MH - Age Factors MH - Animals MH - Aquaculture MH - Blood Chemical Analysis MH - Erythrocyte Count MH - Erythrocyte Indices MH - Female MH - *Hematocrit MH - Leukocyte Count MH - Macrophages, Peritoneal/cytology/*immunology MH - Male MH - Perciformes/*blood/*immunology MH - *Phagocytosis MH - Seasons MH - Sex Factors EDAT- 2012/08/29 06:00 MHDA- 2013/03/05 06:00 CRDT- 2012/08/29 06:00 PHST- 2012/04/04 00:00 [received] PHST- 2012/08/06 00:00 [revised] PHST- 2012/08/08 00:00 [accepted] PHST- 2012/08/29 06:00 [entrez] PHST- 2012/08/29 06:00 [pubmed] PHST- 2013/03/05 06:00 [medline] AID - S1050-4648(12)00290-2 [pii] AID - 10.1016/j.fsi.2012.08.005 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2012 Oct;33(4):953-61. doi: 10.1016/j.fsi.2012.08.005. Epub 2012 Aug 21.