PMID- 22927233
OWN - NLM
STAT- MEDLINE
DCOM- 20130705
LR  - 20211021
IS  - 2042-6984 (Electronic)
IS  - 2042-6976 (Print)
IS  - 2042-6976 (Linking)
VI  - 3
IP  - 1
DP  - 2013 Jan
TI  - Epithelial permeability alterations in an in vitro air-liquid interface model of 
      allergic fungal rhinosinusitis.
PG  - 19-25
LID - 10.1002/alr.21077 [doi]
AB  - BACKGROUND: Chronic rhinosinusitis (CRS) is an inflammatory upper-airway disease 
      with numerous etiologies. Patients with a characteristic subtype of CRS, allergic 
      fungal rhinosinusitis (AFRS), display increased expression of T helper 2 (Th2) 
      cytokines and antigen-specific immunoglobulin E (IgE). Various sinonasal 
      inflammatory conditions are associated with alterations in epithelial barrier 
      function. The aim of this study was to compare epithelial permeability and 
      intercellular junctional protein expression among cultured primary sinonasal 
      cells from AFRS patients vs noninflammatory controls. METHODS: Epithelial cells 
      isolated from paranasal sinus mucosa of AFRS and noninflammatory control patients 
      were grown to confluence on permeable supports and transitioned to air-liquid 
      interface (ALI). Transepithelial resistance (TER) was measured with a horizontal 
      Ussing chamber to characterize the functional permeability of each cell type. 
      After TER recordings were complete, a panel of intercellular junctional proteins 
      was assessed by Western blot and immunofluorescence labeling followed by confocal 
      microscopy. RESULTS: After 12 samples were measured from each group, we observed 
      a 41% mean decrease in TER in AFRS cells (296 +/- 89 ohms x cm(2) ) compared to 
      control (503 +/- 134 ohms x cm(2) , p = 0.006). TER deficits observed in AFRS were 
      associated with decreased expression of the tight junction proteins occludin and 
      junctional adhesion molecule-A (JAM-A), and increased expression of a leaky tight 
      junction protein claudin-2. CONCLUSION: Cultured sinonasal epithelium from AFRS 
      patients displayed increased epithelial permeability and altered expression of 
      intercellular junctional proteins. Given that these cells were not incubated with 
      inflammatory cytokines in vitro, the cultured AFRS epithelial alterations may 
      represent a retained modification in protein expression from the in vivo 
      phenotype.
CI  - Copyright (c) 2013 American Rhinologic Society-American Academy of Otolaryngic 
      Allergy, LLC.
FAU - Den Beste, Kyle A
AU  - Den Beste KA
AD  - Epithelial Pathobiology and Mucosal Inflammation Research Unit, Department of 
      Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30308, USA.
FAU - Hoddeson, Elizabeth K
AU  - Hoddeson EK
FAU - Parkos, Charles A
AU  - Parkos CA
FAU - Nusrat, Asma
AU  - Nusrat A
FAU - Wise, Sarah K
AU  - Wise SK
LA  - eng
GR  - DK072564/DK/NIDDK NIH HHS/United States
GR  - KL2 TR000455/TR/NCATS NIH HHS/United States
GR  - T35-HL007473/HL/NHLBI NIH HHS/United States
GR  - DK061379/DK/NIDDK NIH HHS/United States
GR  - R01 DK072564/DK/NIDDK NIH HHS/United States
GR  - KL2 RR025009/RR/NCRR NIH HHS/United States
GR  - UL1 TR000454/TR/NCATS NIH HHS/United States
GR  - T35 HL007473/HL/NHLBI NIH HHS/United States
GR  - UL1 RR025008/RR/NCRR NIH HHS/United States
GR  - R01 DK059888/DK/NIDDK NIH HHS/United States
GR  - R01 DK061379/DK/NIDDK NIH HHS/United States
GR  - DK059888/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120827
PL  - United States
TA  - Int Forum Allergy Rhinol
JT  - International forum of allergy & rhinology
JID - 101550261
RN  - 0 (Claudin-2)
RN  - 0 (Junctional Adhesion Molecule A)
RN  - 0 (Occludin)
SB  - IM
MH  - Blotting, Western
MH  - Claudin-2/metabolism
MH  - Epithelial Cells/*metabolism
MH  - Humans
MH  - Intercellular Junctions/*metabolism
MH  - Intracellular Membranes/*metabolism
MH  - Junctional Adhesion Molecule A/metabolism
MH  - Microscopy, Confocal
MH  - Occludin/metabolism
MH  - Paranasal Sinuses/*metabolism
MH  - Permeability
MH  - Rhinitis/*metabolism
MH  - Sinusitis/*metabolism
MH  - Tight Junctions/metabolism
PMC - PMC3511593
MID - NIHMS391104
COIS- Conflict of Interest Disclosure: [Table: see text]
EDAT- 2012/08/29 06:00
MHDA- 2013/07/06 06:00
PMCR- 2014/01/01
CRDT- 2012/08/29 06:00
PHST- 2012/02/28 00:00 [received]
PHST- 2012/05/14 00:00 [revised]
PHST- 2012/06/12 00:00 [accepted]
PHST- 2012/08/29 06:00 [entrez]
PHST- 2012/08/29 06:00 [pubmed]
PHST- 2013/07/06 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.1002/alr.21077 [doi]
PST - ppublish
SO  - Int Forum Allergy Rhinol. 2013 Jan;3(1):19-25. doi: 10.1002/alr.21077. Epub 2012 
      Aug 27.