PMID- 22927436
OWN - NLM
STAT- MEDLINE
DCOM- 20121231
LR  - 20231213
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 287
IP  - 42
DP  - 2012 Oct 12
TI  - A viral, transporter associated with antigen processing (TAP)-independent, high 
      affinity ligand with alternative interactions endogenously presented by the 
      nonclassical human leukocyte antigen E class I molecule.
PG  - 34895-34903
LID - S0021-9258(20)62641-2 [pii]
LID - 10.1074/jbc.M112.362293 [doi]
AB  - The transporter associated with antigen processing (TAP) enables the flow of 
      viral peptides generated in the cytosol by the proteasome and other proteases to 
      the endoplasmic reticulum, where they complex with nascent human leukocyte 
      antigen (HLA) class I. Later, these peptide-HLA class I complexes can be 
      recognized by CD8(+) lymphocytes. Cancerous cells and infected cells in which TAP 
      is blocked, as well as individuals with unusable TAP complexes, are able to 
      present peptides on HLA class I by generating them through TAP-independent 
      processing pathways. Here, we identify a physiologically processed HLA-E ligand 
      derived from the D8L protein in TAP-deficient vaccinia virus-infected cells. This 
      natural high affinity HLA-E class I ligand uses alternative interactions to the 
      anchor motifs previously described to be presented on nonclassical HLA class I 
      molecules. This octameric peptide was also presented on HLA-Cw1 with similar 
      binding affinity on both classical and nonclassical class I molecules. In 
      addition, this viral peptide inhibits HLA-E-mediated cytolysis by natural killer 
      cells. Comparison between the amino acid sequences of the presenting HLA-E and 
      HLA-Cw1 alleles revealed a shared structural motif in both HLA class molecules, 
      which could be related to their observed similar cross-reactivity affinities. 
      This motif consists of several residues located on the floor of the 
      peptide-binding site. These data expand the role of HLA-E as an 
      antigen-presenting molecule.
FAU - Lorente, Elena
AU  - Lorente E
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda, Madrid, Spain.
FAU - Infantes, Susana
AU  - Infantes S
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda, Madrid, Spain.
FAU - Abia, David
AU  - Abia D
AD  - Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones 
      Cientificas (CSIC)/Universidad Autonoma de Madrid, 28049 Madrid, Spain.
FAU - Barnea, Eilon
AU  - Barnea E
AD  - Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, 
      Israel.
FAU - Beer, Ilan
AU  - Beer I
AD  - IBM Haifa Research Lab, Haifa 31905, Israel.
FAU - Garcia, Ruth
AU  - Garcia R
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda, Madrid, Spain.
FAU - Lasala, Fatima
AU  - Lasala F
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda, Madrid, Spain.
FAU - Jimenez, Mercedes
AU  - Jimenez M
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda, Madrid, Spain.
FAU - Mir, Carmen
AU  - Mir C
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda, Madrid, Spain.
FAU - Morreale, Antonio
AU  - Morreale A
AD  - Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones 
      Cientificas (CSIC)/Universidad Autonoma de Madrid, 28049 Madrid, Spain.
FAU - Admon, Arie
AU  - Admon A
AD  - Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, 
      Israel.
FAU - Lopez, Daniel
AU  - Lopez D
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda, Madrid, Spain. Electronic address: dlopez@isciii.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120827
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antigens, Viral)
RN  - 0 (D8L protein, vaccinia virus)
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-Cw1 antigen)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptides)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (transporter associated with antigen processing (TAP))
SB  - IM
MH  - ATP-Binding Cassette Transporters/genetics/*immunology/metabolism
MH  - Amino Acid Motifs
MH  - *Antigen Presentation
MH  - Antigens, Viral/genetics/*immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cell Line
MH  - HLA-C Antigens/genetics/immunology/metabolism
MH  - Histocompatibility Antigens Class I/genetics/*immunology/metabolism
MH  - Humans
MH  - Membrane Proteins/genetics/*immunology/metabolism
MH  - Peptides/genetics/immunology/metabolism
MH  - Vaccinia/genetics/*immunology/metabolism
MH  - Vaccinia virus/genetics/*immunology/metabolism
MH  - Viral Structural Proteins/genetics/*immunology/metabolism
MH  - HLA-E Antigens
PMC - PMC3471699
EDAT- 2012/08/29 06:00
MHDA- 2013/01/01 06:00
PMCR- 2013/10/12
CRDT- 2012/08/29 06:00
PHST- 2012/08/29 06:00 [entrez]
PHST- 2012/08/29 06:00 [pubmed]
PHST- 2013/01/01 06:00 [medline]
PHST- 2013/10/12 00:00 [pmc-release]
AID - S0021-9258(20)62641-2 [pii]
AID - M112.362293 [pii]
AID - 10.1074/jbc.M112.362293 [doi]
PST - ppublish
SO  - J Biol Chem. 2012 Oct 12;287(42):34895-34903. doi: 10.1074/jbc.M112.362293. Epub 
      2012 Aug 27.