PMID- 22927773
OWN - NLM
STAT- MEDLINE
DCOM- 20130108
LR  - 20220331
IS  - 1449-1907 (Electronic)
IS  - 1449-1907 (Linking)
VI  - 9
IP  - 6
DP  - 2012
TI  - Osteogenic differentiation of dental follicle stem cells.
PG  - 480-7
LID - 10.7150/ijms.4583 [doi]
AB  - BACKGROUND: Stem cells are defined as clonogenic cells capable of self-renewal 
      and multi-lineage differentiation. A population of these cells has been 
      identified in human Dental Follicle (DF). Dental Follicle Stem Cells (DFSCs) were 
      found in pediatric unerupted wisdom teeth and have been shown to differentiate, 
      under particular conditions, into various cell types of the mesenchymal tissues. 
      AIM: The aim of this study was to investigate if cells isolated from DF show stem 
      features, differentiate toward osteoblastic phenotype and express osteoblastic 
      markers. METHODS: We studied the immunophenotype of DFSCs by flow cytometric 
      analysis, the osteoblastic markers of differentiated DFSCs were assayed by 
      histochemical methods and real-time PCR. RESULTS: We demonstrated that DFSCs 
      expressed a heterogeneous assortment of makers associated with stemness. Moreover 
      DFSCs differentiated into osteoblast-like cells, producing mineralized matrix 
      nodules and expressed the typical osteoblastic markers, Alkaline Phosphatase 
      (ALP) and Collagen I (Coll I). CONCLUSION: This study suggests that DFSCs may 
      provide a cell source for tissue engineering of bone.
FAU - Mori, Giorgio
AU  - Mori G
AD  - Department of Biomedical Sciences, Medical School, University of Foggia, Italy. 
      g.mori@unifg.it
FAU - Ballini, Andrea
AU  - Ballini A
FAU - Carbone, Claudia
AU  - Carbone C
FAU - Oranger, Angela
AU  - Oranger A
FAU - Brunetti, Giacomina
AU  - Brunetti G
FAU - Di Benedetto, Adriana
AU  - Di Benedetto A
FAU - Rapone, Biagio
AU  - Rapone B
FAU - Cantore, Stefania
AU  - Cantore S
FAU - Di Comite, Mariasevera
AU  - Di Comite M
FAU - Colucci, Silvia
AU  - Colucci S
FAU - Grano, Maria
AU  - Grano M
FAU - Grassi, Felice R
AU  - Grassi FR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120813
PL  - Australia
TA  - Int J Med Sci
JT  - International journal of medical sciences
JID - 101213954
RN  - 0 (Collagen Type I)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Cell Differentiation/genetics/*physiology
MH  - Cells, Cultured
MH  - Child
MH  - Collagen Type I/metabolism
MH  - Dental Sac/*cytology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - Male
MH  - Osteogenesis/genetics/physiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Stem Cells/*cytology/metabolism
PMC - PMC3427952
OTO - NOTNLM
OT  - bone tissue engineering
OT  - dental follicle
OT  - human postnatal dental tissue.
OT  - osteogenic markers
OT  - stem cells
COIS- CONFLICT OF INTEREST: The authors have declared that no competing interest 
      exists.
EDAT- 2012/08/29 06:00
MHDA- 2013/01/09 06:00
PMCR- 2012/01/01
CRDT- 2012/08/29 06:00
PHST- 2012/05/12 00:00 [received]
PHST- 2012/07/31 00:00 [accepted]
PHST- 2012/08/29 06:00 [entrez]
PHST- 2012/08/29 06:00 [pubmed]
PHST- 2013/01/09 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - ijmsv09p0480 [pii]
AID - 10.7150/ijms.4583 [doi]
PST - ppublish
SO  - Int J Med Sci. 2012;9(6):480-7. doi: 10.7150/ijms.4583. Epub 2012 Aug 13.