PMID- 22929654
OWN - NLM
STAT- MEDLINE
DCOM- 20130314
LR  - 20121002
IS  - 1879-291X (Electronic)
IS  - 0301-5629 (Linking)
VI  - 38
IP  - 11
DP  - 2012 Nov
TI  - Disruption of splenic circulation using microbubble-enhanced ultrasound and 
      prothrombin: a preliminary study.
PG  - 1930-7
LID - S0301-5629(12)00429-2 [pii]
LID - 10.1016/j.ultrasmedbio.2012.07.012 [doi]
AB  - The spleen is a solid organ in which splenomegaly frequently develops and to 
      which abdominal blunt trauma occurs. In this study, we demonstrated the potential 
      therapeutic effect of microbubble-enhanced ultrasound (MEUS) combined with 
      prothrombin to disrupt splenic circulation. A high-pressure-amplitude therapeutic 
      ultrasound (TUS) device was used to treat 36 surgically exposed spleens in 
      healthy New Zealand rabbits. Eighteen spleens were treated with either MEUS (n = 
      9) or MEUS combined with prothrombin (n = 9). The other 18 spleens were treated 
      with TUS only or sham ultrasound exposure and served as the controls. The TUS was 
      operated at a frequency of 831 kHz and a peak negative pressure of 4.8 MPa. 
      Prothrombin was administered intravenously at 20 IU/kg. Contrast-enhanced 
      ultrasound (CEUS) and acoustic quantification were performed to assess splenic 
      blood perfusion. We found significant blood perfusion slowdown and drop-off in 
      the MEUS-treated spleens. The peak intensity dropped from 20.2 +/- 2.70 dB to 11.6 
      +/- 4.58 dB immediately after treatment. The spleens treated with the combination 
      of MEUS and prothrombin showed consistently poor perfusion within 1 h. In 
      histologic examination of the MEUS-treated spleens, we found significant 
      dilatation of splenic sinuses, hemorrhage, interstitial edema and thrombosis. 
      This study demonstrated that the vascular effects induced by 
      microbubble-enhanced, high-pressure ultrasound can slow down or block blood 
      perfusion in the rabbit spleen. Prothrombin helps to enhance and extend the 
      effects for up to 1 h.
CI  - Copyright (c) 2012 World Federation for Ultrasound in Medicine & Biology. Published 
      by Elsevier Inc. All rights reserved.
FAU - Liu, Jianhua
AU  - Liu J
AD  - Department of Function, Guangzhou First Municipal People's Hospital, Guangzhou 
      Medical College, Guangzhou, China.
FAU - Xiao, Shuyi
AU  - Xiao S
FAU - Wu, Shengzheng
AU  - Wu S
FAU - Ou, Wencai
AU  - Ou W
FAU - He, Jingguang
AU  - He J
FAU - Gao, Shunji
AU  - Gao S
FAU - Liu, Zheng
AU  - Liu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120825
PL  - England
TA  - Ultrasound Med Biol
JT  - Ultrasound in medicine & biology
JID - 0410553
RN  - 9001-26-7 (Prothrombin)
SB  - IM
MH  - Animals
MH  - Blood Flow Velocity/drug effects/physiology/radiation effects
MH  - High-Intensity Focused Ultrasound Ablation/*methods
MH  - Microbubbles/therapeutic use
MH  - Pilot Projects
MH  - Prothrombin/*therapeutic use
MH  - Rabbits
MH  - Radiation Tolerance/*drug effects
MH  - Splenic Artery/drug effects/*physiopathology/*radiation effects
MH  - Treatment Outcome
MH  - Ultrasonography/methods
EDAT- 2012/08/30 06:00
MHDA- 2013/03/15 06:00
CRDT- 2012/08/30 06:00
PHST- 2011/11/09 00:00 [received]
PHST- 2012/07/10 00:00 [revised]
PHST- 2012/07/18 00:00 [accepted]
PHST- 2012/08/30 06:00 [entrez]
PHST- 2012/08/30 06:00 [pubmed]
PHST- 2013/03/15 06:00 [medline]
AID - S0301-5629(12)00429-2 [pii]
AID - 10.1016/j.ultrasmedbio.2012.07.012 [doi]
PST - ppublish
SO  - Ultrasound Med Biol. 2012 Nov;38(11):1930-7. doi: 
      10.1016/j.ultrasmedbio.2012.07.012. Epub 2012 Aug 25.