PMID- 22930579
OWN - NLM
STAT- MEDLINE
DCOM- 20130129
LR  - 20151119
IS  - 1552-4930 (Electronic)
IS  - 1552-4922 (Linking)
VI  - 81
IP  - 10
DP  - 2012 Oct
TI  - Type 2 diabetes mellitus impairs the maturation of endothelial progenitor cells 
      and increases the number of circulating endothelial cells in peripheral blood.
PG  - 856-64
LID - 10.1002/cyto.a.22109 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is associated with endothelial dysfunction, which 
      leads to vascular complications. Endothelial progenitor cells (EPCs) are thought 
      to be a subset of cells derived from the bone marrow that play a crucial role in 
      the neovascularization of ischemic tissue and in the maintenance of endothelial 
      cell integrity. In contrast, circulating endothelial cells (CECs) are of 
      endothelial origin and become detached from the intima of blood vessels in 
      response to pathological stimuli. The study investigated the effects of T2DM on 
      subpopulations of EPCs and CECs in peripheral blood, as compared with the effects 
      on unacylated (UAG) and acylated (AG) ghrelin levels, which have been shown 
      recently to play an important role in endothelial dysfunction associated with 
      diabetes. Using the high-performance flow cytometer FACSCanto, and UAG/AG ghrelin 
      enzyme immunoassay kits, we analyzed whole peripheral blood samples from: (i) 
      diabetic patients with a history of disease of less than 1 year and no clinical 
      evidence of angiopathy, (ii) diabetic patients with long-standing disease with 
      vascular complications, and (iii) healthy donors. We found that T2DM did not 
      affect bone-marrow mobilization, but it altered the UAG/AG profile and decreased 
      the number of highly differentiated EPCs (late EPCs) greatly. In addition, T2DM 
      increased the number of CECs, together with the number of activated CECs. Our 
      results suggest that: (i) the endothelial damage could be due mainly to altered 
      maturation/commitment of EPCs, rather than a simple decrease in their production 
      in the bone marrow; and (ii) EPC subpopulations and ghrelin levels could be 
      useful markers to assess endothelial damage in diabetes.
CI  - Copyright (c) 2012 International Society for Advancement of Cytometry.
FAU - Lombardo, Marco F
AU  - Lombardo MF
AD  - Centro Unico Regionale Trapianti Midollo Osseo e Terapie Cellulari Alberto Neri, 
      Azienda Ospedaliera Bianchi-Melacrino-Morelli, 89100 Reggio Calabria, Italy.
FAU - Iacopino, Pasquale
AU  - Iacopino P
FAU - Cuzzola, Maria
AU  - Cuzzola M
FAU - Spiniello, Elisa
AU  - Spiniello E
FAU - Garreffa, Cristina
AU  - Garreffa C
FAU - Ferrelli, Francesca
AU  - Ferrelli F
FAU - Coppola, Andrea
AU  - Coppola A
FAU - Saccardi, Riccardo
AU  - Saccardi R
FAU - Piaggesi, Alberto
AU  - Piaggesi A
FAU - Piro, Rosanna
AU  - Piro R
FAU - Mannino, Domenico
AU  - Mannino D
FAU - Grossi, Giovanni
AU  - Grossi G
FAU - Lauro, Davide
AU  - Lauro D
FAU - Irrera, Giuseppe
AU  - Irrera G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120828
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
RN  - 0 (Biomarkers)
RN  - 0 (Ghrelin)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Acylation
MH  - Biomarkers/blood
MH  - Bone Marrow/immunology/pathology
MH  - Case-Control Studies
MH  - Cell Count
MH  - Cell Differentiation/immunology
MH  - Diabetes Mellitus, Type 2/blood/complications/immunology/*pathology
MH  - Diabetic Retinopathy/blood/etiology/immunology/*pathology
MH  - Endothelial Cells/immunology/*pathology
MH  - Endothelium, Vascular/immunology/*pathology
MH  - Female
MH  - Flow Cytometry
MH  - Ghrelin/blood
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Middle Aged
MH  - Protein Isoforms/blood
MH  - Stem Cells/immunology/*pathology
MH  - Tunica Intima/immunology/*pathology
EDAT- 2012/08/30 06:00
MHDA- 2013/01/30 06:00
CRDT- 2012/08/30 06:00
PHST- 2011/10/28 00:00 [received]
PHST- 2012/06/24 00:00 [revised]
PHST- 2012/06/28 00:00 [accepted]
PHST- 2012/08/30 06:00 [entrez]
PHST- 2012/08/30 06:00 [pubmed]
PHST- 2013/01/30 06:00 [medline]
AID - 10.1002/cyto.a.22109 [doi]
PST - ppublish
SO  - Cytometry A. 2012 Oct;81(10):856-64. doi: 10.1002/cyto.a.22109. Epub 2012 Aug 28.