PMID- 22931638
OWN - NLM
STAT- MEDLINE
DCOM- 20130509
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 20
IP  - 4
DP  - 2012 Aug
TI  - [Effect of knocking down eEF1A1 gene on proliferation and apoptosis in Jurkat 
      cells and its mechanisms].
PG  - 835-41
AB  - This study was purposed to investigate the effect of knocking down eukaryotic 
      elongation factor 1A1 (eEF1A1) gene on the proliferation and apoptosis in human 
      acute T lymphocytic leukemia (T-ALL) cell line Jurkat and explore its mechanism. 
      The eEF1A1 mRNA and protein expressions of Jurkat cells and 3 healthy adult 
      peripheral blood mononuclear cells (PBMNC) were detected by real time PCR and 
      Western blot, respectively. eEF1A1-shRNA lentivirus was constructed through 
      molecular biological method, and was used to transfect Jurkat cells. Then, cell 
      eEF1A1 mRNA and protein expressions were detected by real time PCR and Western 
      blot, respectively. Cell proliferation, apoptosis and cycle were detected by MTT 
      method, Annexin V-APC labeling and DNA ploidy analysis, respectively. 
      Cell-related protein expressions of phosphatidylinositol-3-kinase 
      (PI3K)/serine/threonine kinase (Akt) signaling pathway were detected by Western 
      blot. The results showed that eEF1A1 mRNA and protein expression levels of Jurkat 
      cells were significantly higher than that of healthy adult PBMNC, respectively (P 
      < 0.01, P < 0.05). eEF1A1 mRNA and protein expressions of Jurkat cells were 
      significantly knocked down by constructing eEF1A1-shRNA lentivirus. Compared to 
      negative control group (transfected with negative control-shRNA lentivirus), cell 
      proliferation in eEF1A1-shRNA group was significantly inhibited, cell apoptosis 
      was remarkably induced, cell cycle was blocked in G(0)/G(1) phase, and the 
      expression levels of p-Akt (Ser 473), nuclear factor kappa B (NF-kappaB), p-NF-kappaB 
      (Ser 468), mammalian target of rapamycin (mTOR) and p-mTOR (Ser 2448) proteins 
      were significantly reduced. It is concluded that eEF1A1 may be a putative 
      oncoprotein in T-ALL cells. Knocking down eEF1A1 gene has noticeable effects on 
      the proliferation inhibition and apoptosis induction of Jurkat cells, which may 
      be mediated by the down-regulation of PI3K/Akt/NF-kappaB and PI3K/Akt/mTOR signaling 
      pathway.
FAU - Huang, Yi
AU  - Huang Y
AD  - Department of Clinical Laboratory, Provincial Clinical College, Fujian Medical 
      University, Fujian Province, China. Hyi8070@126.com
FAU - Hu, Jian-Da
AU  - Hu JD
FAU - Qi, Yuan-Lin
AU  - Qi YL
FAU - Wu, Yan-An
AU  - Wu YA
FAU - Zheng, Jing
AU  - Zheng J
FAU - Chen, Ying-Yu
AU  - Chen YY
FAU - Huang, Xiao-Li
AU  - Huang XL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (EEF1A1 protein, human)
RN  - 0 (Peptide Elongation Factor 1)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - *Apoptosis
MH  - *Cell Proliferation
MH  - Gene Silencing
MH  - Humans
MH  - Jurkat Cells
MH  - Peptide Elongation Factor 1/*genetics
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction/genetics
EDAT- 2012/08/31 06:00
MHDA- 2013/05/10 06:00
CRDT- 2012/08/31 06:00
PHST- 2012/08/31 06:00 [entrez]
PHST- 2012/08/31 06:00 [pubmed]
PHST- 2013/05/10 06:00 [medline]
AID - 1009-2137(2012)04-0835-07 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Aug;20(4):835-41.