PMID- 22932142
OWN - NLM
STAT- MEDLINE
DCOM- 20130312
LR  - 20120830
IS  - 1673-0860 (Print)
IS  - 1673-0860 (Linking)
VI  - 47
IP  - 6
DP  - 2012 Jun
TI  - [Expressions and clinical significance of high mobility group box-1 mRNA and 
      protein in laryngeal squamous cell carcinoma tissues and serum].
PG  - 487-90
AB  - OBJECTIVE: To investigate the mRNA and protein expressions of high mobility group 
      box-1 (HMGB1) in the tumor tissues and sera of patients with laryngeal squamous 
      cell carcinoma (LSCC) and their clinical significance. METHODS: Semi-quantitative 
      reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were 
      used to detected the expressions of HMGB1 mRNA and protein in the tumors and 
      adjacent normal epithelial tissues in 30 patients with LSCC. Serum HMGB1 protein 
      levels in the patients with LSCC and in 10 healthy volunteers were detected with 
      enzyme-linked immunosorbent adsorption experiment (ELISA). RESULTS: RT-PCR 
      demonstrated that the mean relative mRNA expression levels of HMGB1 (HMGB1/GAPDH) 
      in LSCC tissues and in adjacent normal epithelial tissues were 1.25 +/- 0.12 and 
      0.32 +/- 0.04, respectively (t = 40.27, P < 0.05). Western blot revealed that the 
      mean relative protein expression levels of HMGB1 (HMGB1/beta-actin) were 1.29 +/- 0.10 
      and 0.34 +/- 0.03 (t = 49.84, P < 0.05), respectively. Both mRNA and protein 
      expression levels of HMGB1 were associated with T stage, clinical stage, lymph 
      node metastasis status and smoking (all P < 0.05), but no significant correlation 
      with age, alcohol consumption and primary tumor grade and location (all P > 
      0.05). Mean serum HMGB1 protein levels in patients with LSCC and healthy 
      volunteers were (24.80 +/- 14.08) ng/ml and (23.58 +/- 14.69) ng/ml (t = 0.37, P > 
      0.05). CONCLUSIONS: Both mRNA and protein expressions of HMGB1 were obviously 
      elevated in LSCC, which were associated closely with T stage, clinical stage and 
      lymph node metastasis.
FAU - Guo, Ying
AU  - Guo Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital, 
      Central South University, Changsha 410008, China.
FAU - Liu, Yong
AU  - Liu Y
FAU - Tan, Ping-qing
AU  - Tan PQ
FAU - Li, Guo
AU  - Li G
FAU - Su, Zhong-wu
AU  - Su ZW
FAU - Tian, Yong-quan
AU  - Tian YQ
FAU - Qiu, Yuan-zheng
AU  - Qiu YZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of 
      otorhinolaryngology head and neck surgery
JID - 101247574
RN  - 0 (HMGB1 Protein)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/blood/*metabolism/pathology
MH  - Case-Control Studies
MH  - Female
MH  - HMGB1 Protein/blood/genetics/*metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/blood/*metabolism/pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - RNA, Messenger/blood/genetics/metabolism
EDAT- 2012/08/31 06:00
MHDA- 2013/03/13 06:00
CRDT- 2012/08/31 06:00
PHST- 2012/08/31 06:00 [entrez]
PHST- 2012/08/31 06:00 [pubmed]
PHST- 2013/03/13 06:00 [medline]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Jun;47(6):487-90.