PMID- 22932403
OWN - NLM
STAT- MEDLINE
DCOM- 20130314
LR  - 20181201
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 41
IP  - 6
DP  - 2012 Jun
TI  - [Detection of chromosomal translocations involving ALK gene in anaplastic large 
      cell lymphoma by fluorescence in-situ hybridization and its clinical 
      significance].
PG  - 371-5
LID - 10.3760/cma.j.issn.0529-5807.2012.06.003 [doi]
AB  - OBJECTIVE: To investigate clinicopathologic features and clinical value of the 
      chromosomal translocation involving anaplastic lymphoma kinase (ALK) in 
      anaplastic large cell lymphoma (ALCL) by fluorescence in situ hybridization 
      (FISH). METHODS: A total of 55 cases, including 45 cases of ALCL and 10 reactive 
      lymphoid hyperplasia, were collected during 1999 to 2006 in the Department of 
      Pathology, Fudan University Shanghai Cancer Center, and Xinhua Hospital 
      Affiliated to Shanghai Jiaotong University. All cases were studied by FISH using 
      dual color break apart probes of ALK for detection of chromosomal translocation, 
      compared with the previous results of immunohistochemistry (IHC) and 
      reverse-transcriptase polymerase chain reaction (RT-PCR) for the detection of ALK 
      aberrations. RESULTS: The result of FISH showed that the clear red and green 
      fluorescence signals were detected in 38 cases of ALCL, in which conspicuous 
      split signals were observed in tumor cells in 24 cases (63.2%), suggesting the 
      rearrangement of the ALK locus, with multiple copies of ALK gene in one case. In 
      addition, the rearrangement of the ALK locus was not identified in 14 of 38 cases 
      (36.8%); and the FISH results were unable to be evaluated in 7 cases, because no 
      fluorescent signals involving ALK gene were found or signals were too weak to be 
      analyzed. The concordance for the detection ALK aberrations in ALCL between FISH 
      and RT-PCR, FISH and IHC were both statistically significant (P < 0.01). 
      Chromosomal translocation involving ALK gene was not found in all 10 cases of 
      reactive lymphoid hyperplasia. CONCLUSIONS: ALCL is an entity of lymphoma 
      characterized by special clinical presentation, morphology, and ALK aberrations. 
      FISH is helpful for detection of the chromosomal translocations involving ALK in 
      ALCL, however, the detection efficiency by FISH may be affected by storage time 
      of the paraffin-embedded tissue; and therefore combined detection with IHC and 
      RT-PCR could complement each other and help for differential diagnosis of 
      ALK(+)ALCL from ALK(-)ALCL.
FAU - Huang, Wen-tao
AU  - Huang WT
AD  - Department of Pathology, Fudan University Shanghai Cancer Center, China.
FAU - Li, Xiao-qiu
AU  - Li XQ
FAU - Yao, Xiao-hong
AU  - Yao XH
FAU - Lu, Yong-ming
AU  - Lu YM
FAU - Sheng, Wei-qi
AU  - Sheng WQ
FAU - Lu, Hong-fen
AU  - Lu HF
FAU - Zhou, Xiao-yan
AU  - Zhou XY
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Anaplastic Lymphoma Kinase
MH  - Child
MH  - Child, Preschool
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Large-Cell, Anaplastic/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Receptor Protein-Tyrosine Kinases/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Translocation, Genetic
MH  - Young Adult
EDAT- 2012/08/31 06:00
MHDA- 2013/03/15 06:00
CRDT- 2012/08/31 06:00
PHST- 2012/08/31 06:00 [entrez]
PHST- 2012/08/31 06:00 [pubmed]
PHST- 2013/03/15 06:00 [medline]
AID - 10.3760/cma.j.issn.0529-5807.2012.06.003 [doi]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2012 Jun;41(6):371-5. doi: 
      10.3760/cma.j.issn.0529-5807.2012.06.003.