PMID- 22932408
OWN - NLM
STAT- MEDLINE
DCOM- 20130314
LR  - 20181023
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 41
IP  - 6
DP  - 2012 Jun
TI  - [Down-regulation of histone deacetylase 2 induces cell apoptosis and inhibits 
      cell proliferation and migration of laryngeal squamous cell carcinoma cells].
PG  - 396-9
LID - 10.3760/cma.j.issn.0529-5807.2012.06.008 [doi]
AB  - OBJECTIVE: To investigate the effects of histone deacetylase 2 (HDAC2) expression 
      on cell proliferation, apoptosis and migration of laryngeal squamous cell 
      carcinoma (LSCC) Hep-2 cells. METHODS: HDAC2 siRNA and control siRNA were 
      transfected into LSCC Hep-2 cells by lipofectamine 2000, and cells were divided 
      into three experimental groups: untreated group, control siRNA group and HDAC2 
      siRNA transfection group. Western blotting was utilized to detect the expression 
      of HDAC2 protein in Hep-2 cells. Cell proliferation and apoptosis were 
      investigated by CCK-8 kit and flow cytometry, respectively. Boyden chamber was 
      used to study cell migration. Expressions of cell apoptosis and cell migration 
      related proteins were detected by Western blotting. RESULTS: HDAC2 siRNA 
      significantly down-regulated the expression of HDAC2 protein in LSCC Hep-2 cells. 
      Down-regulation of HDAC2 expression coincided with an inhibition of cell 
      proliferation and migration along with an induced cell apoptosis of Hep-2 cells. 
      Moreover, down-regulation of HDAC2 expression significantly increased the 
      expressions of caspase-3 and caspase-9 proteins but decreased the expressions of 
      matrix metalloproteinases (MMP)-2 and MMP-9 proteins. CONCLUSIONS: HDAC2 may play 
      a pivotal role in the initiation and development of LSCC. Down-regulation of 
      HDAC2 expression mediates cell apoptosis. Cell migration inhibition may be 
      tightly associated with overexpression of caspase-3 and caspase-9 along with 
      down-regulation of MMP-2 and MMP-9 expressions.
FAU - Yuan, Lin-lin
AU  - Yuan LL
AD  - Department of Otorhinolaryngology, the First Affiliated Hospital of Zhengzhou 
      University, China.
FAU - Lou, Wei-hua
AU  - Lou WH
FAU - Sang, Jian-zhong
AU  - Sang JZ
FAU - Li, Sheng-lei
AU  - Li SL
FAU - Li, Wen-cai
AU  - Li WC
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (CASP9 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 9)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.5.1.98 (HDAC2 protein, human)
RN  - EC 3.5.1.98 (Histone Deacetylase 2)
SB  - IM
MH  - *Apoptosis
MH  - Carcinoma, Squamous Cell/metabolism/*pathology
MH  - Caspase 3/metabolism
MH  - Caspase 9/metabolism
MH  - Cell Line, Tumor
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Down-Regulation
MH  - Histone Deacetylase 2/genetics/*metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/metabolism/*pathology
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - Transfection
EDAT- 2012/08/31 06:00
MHDA- 2013/03/15 06:00
CRDT- 2012/08/31 06:00
PHST- 2012/08/31 06:00 [entrez]
PHST- 2012/08/31 06:00 [pubmed]
PHST- 2013/03/15 06:00 [medline]
AID - 10.3760/cma.j.issn.0529-5807.2012.06.008 [doi]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2012 Jun;41(6):396-9. doi: 
      10.3760/cma.j.issn.0529-5807.2012.06.008.