PMID- 22933664
OWN - NLM
STAT- MEDLINE
DCOM- 20130125
LR  - 20200226
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 93
IP  - Pt 12
DP  - 2012 Dec
TI  - Identification of a novel B-cell epitope of Hantaan virus glycoprotein recognized 
      by neutralizing 3D8 monoclonal antibody.
PG  - 2595-2600
LID - 10.1099/vir.0.045302-0 [doi]
AB  - Hantaan virus (HTNV), a member of the family Bunyaviridae, is a major agent 
      causing haemorrhagic fever with renal syndrome, a high-mortality-rate disease 
      threatening approximately 150 000 people around the world yearly. The 3D8 mAb 
      displays a neutralizing activity to HTNV infection. In this study, the B-cell 
      epitopes of HTNV glycoproteins (GPs) were finely mapped by peptide scanning. A 
      new B-cell epitope (882)GFLCPEFPGSFRKKC(896) of HTNV, which locates on Gc, has 
      been screened out from a set of 15-mer synthesized peptides covering the 
      full-length of HTNV-GPs. It has been shown by the alanine-scanning technique that 
      (885)C, (893)R, (894)K, (895)K and (896)C are the key amino acids of the binding 
      sites of the GPs. The implications of identifying a novel B-cell epitope for 
      hantavirus immunology and vaccinology are discussed.
FAU - Yan, Guolin
AU  - Yan G
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Zhang, Yusi
AU  - Zhang Y
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Ma, Ying
AU  - Ma Y
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Yi, Jing
AU  - Yi J
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Liu, Bei
AU  - Liu B
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Xu, Zhuwei
AU  - Xu Z
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Zhang, Yun
AU  - Zhang Y
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Zhang, Chunmei
AU  - Zhang C
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Zhang, Fanglin
AU  - Zhang F
AD  - Department of Microbiology, the Fourth Military Medical University, 17 Changle 
      West Road, Xi'an, Shaanxi 710032, PR China.
FAU - Xu, Zhikai
AU  - Xu Z
AD  - Department of Microbiology, the Fourth Military Medical University, 17 Changle 
      West Road, Xi'an, Shaanxi 710032, PR China.
FAU - Yang, Angang
AU  - Yang A
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Zhuang, Ran
AU  - Zhuang R
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
FAU - Jin, Boquan
AU  - Jin B
AD  - Department of Immunology, the Fourth Military Medical University, 17 Changle West 
      Road, Xi'an, Shaanxi 710032, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120829
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Neutralizing/immunology
MH  - Antibodies, Viral/immunology
MH  - Antibody Specificity
MH  - Binding Sites/genetics
MH  - Epitope Mapping
MH  - Epitopes, B-Lymphocyte/genetics/*immunology
MH  - Glycoproteins/genetics/immunology
MH  - Hantaan virus/genetics/*immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Viral Proteins/genetics/immunology
EDAT- 2012/08/31 06:00
MHDA- 2013/01/26 06:00
CRDT- 2012/08/31 06:00
PHST- 2012/08/31 06:00 [entrez]
PHST- 2012/08/31 06:00 [pubmed]
PHST- 2013/01/26 06:00 [medline]
AID - 10.1099/vir.0.045302-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2012 Dec;93(Pt 12):2595-2600. doi: 10.1099/vir.0.045302-0. Epub 2012 
      Aug 29.