PMID- 22934417
OWN - NLM
STAT- MEDLINE
DCOM- 20130905
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 5
DP  - 2012 May
TI  - Adsorption behavior of plasmid DNA onto perfusion chromatographic matrix.
PG  - 522-6
AB  - Anion exchange chromatography is the most popular chromatographic method for 
      plasmid separation. POROS RI 50 is a perfusion chromatographic support which is a 
      reversed phase matrix and is an alternative to conventional ones due to its mass 
      transfer properties. The adsorption and elution of the pIDKE2 plasmid onto 
      reversed phase POROS R1 50 was studied. Langmuir isotherm model was adjusted in 
      order to get the maximum adsorption capacity and the dissociation constant for 
      POROS R1 50-plasmid DNA (pDNA) system. Breakthrough curves were obtained for 
      volumetric flows between 0.69-3.33 mL/min, given dynamic capacity up to 2.3 times 
      higher than those reported for ionic exchange matrix used during the purification 
      process of plasmids with similar size to that of pIDKE2. The efficiency was less 
      than 45% for the flow conditions and initial concentration studied, which means 
      that the support will not be operated under saturation circumstances.
FAU - Limonta, Miladys
AU  - Limonta M
AD  - Center for Genetic Engineering and Biotechnology, Habana 10600, Cuba. 
      miladys.limonta@cigb.edu.cu
FAU - Zumalacarregui, Lourdes
AU  - Zumalacarregui L
FAU - Soler, Dayana
AU  - Soler D
LA  - eng
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anion Exchange Resins)
RN  - 0 (Anions)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Anion Exchange Resins/chemistry
MH  - Anions/chemistry
MH  - Chromatography, Ion Exchange/instrumentation/*methods
MH  - Chromatography, Reverse-Phase/instrumentation/*methods
MH  - DNA/chemistry/*isolation & purification
MH  - Plasmids/chemistry/*isolation & purification
MH  - Porosity
EDAT- 2012/09/01 06:00
MHDA- 2013/09/06 06:00
CRDT- 2012/09/01 06:00
PHST- 2012/09/01 06:00 [entrez]
PHST- 2012/09/01 06:00 [pubmed]
PHST- 2013/09/06 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.11045 [doi]
PST - ppublish
SO  - Se Pu. 2012 May;30(5):522-6. doi: 10.3724/sp.j.1123.2011.11045.